糖尿病肾病患者血清miRNA377的表达及其对SM AD信号通路调控作用

目的：探索糖尿病肾病（DN）患者血清miRNA377（miR377）的表达特点及其可能的下游调控通道。方法用实时荧光定量PCR方法检测经肾活检确诊的DN组、糖尿病无肾病损害（DM ）组和健康人群（对照组）血清中miR377的相对表达量，用TargetScan等生物信息学的方法预测miR377调节的靶基因，并在体外细胞模型中上调miR377的表达，检测下游机制蛋白。结果 DN组（1．32±0．13）和DM 组（0．92±0．11）患者血清中miR‐377均高于对照组（0．33±0．06），差异有统计学意义（P＜0．01）；生物信息学分析显示为糖尿病肾病中miR377的靶基因为SMAD ；Western blot结果显示，转染miR377 mimics组HMC细胞中SMAD2和SMAD3蛋白的表达量均高于miR377 NC组和对照组（P＜0．05），而SMAD7在各组中表达量无明显差异（P＞0．05）。结论糖尿病肾病患者血清的miR377存在异常高表达，可能预示肾脏病变的程度。

Mirna377 expression in serum of patients with diabetic nephropathy and the role of SMAD signaling pathway

Objective To explore the characteristics of patie nts with diabetic nephropathy serum miRNA377(miR377) ex‐pression and the possible regulation of downstream channels .Methods Real‐time PCR method to detect biopsy‐confirmed DN group ,diabetes without kidney damage group (DM ) and serum of healthy people relative expression of miR377 ,with the Tar‐getScan bioinformatics method for predicting the miR377 target gene regulation and raised miR377 expression in vitro model ,the detection mechanism downstream protein .Results Compared with healthy control patients(0 .33 ± 0 .06) ,patients with diabetic ne‐phropathy(1 .32 ± 0 .13) in serum miR377 expression (P<0 .01) ,diabetes without kidney damage group(0 .92 ± 0 .11) miR377 is also highly expressed (P<0 .01) ,bioinformatics analysis showed that diabetic nephropathy the miR377 target genes of SMAD ;Western blot showed transfected miR377 mimics group HMC cells SMAD2 and SMAD3 protein expression levels higher than miR377 NC group and the control group (P<0 .05) ,while SMAD7 The expression in each group had no significant difference (P>0 .05) amount .Conclusion The patients with diabetic nephropathy serum miR377 expression exists abnormally high level ,may in‐dicate kidney disease ,the mechanism may be activated TGF‐β /Smad pathway and increased expression of SMAD2 SMAD3 protein expression .