VES对人胃癌细胞中细胞周期调控因子蛋白表达的影响

本研究采用免疫细胞化学方法检测了维生素Ｅ琥珀酸酯（ＶＥＳ）处理人胃癌（ＳＧＣ－７９０１）细胞４８小时后某些细胞周期调控因子及细胞凋亡调控基因的蛋白表达。结果表明ＶＥＳ能明显抑制ＳＧＣ－７９０１细胞的ｐ１６、ｐ５３、ｐ２１和ＰＣＮＡ的蛋白表达并均有明显的剂量－效应关系。以ｐ１６的降低作用最为明显，依次顺序为ｐ２１、ｐ５３和ＰＣＮＡ。与此相反，在ＶＥＳ处理后ｃ－ｆｏｓ蛋白表达被明显诱导。上述结果提示在离体试验条件下ＶＥＳ具有潜在的抗癌作用。

The effects of vitamin E succinate on the protein expression of cell cycle regulation factors in human gastric carcinoma cells.

Using immunocytochemistry technique, the protein expression of some cell cycle regulation factors and apoptosis regulation genes was determined in human gastric carcinoma (SGC 7901) cells treated with Vitamin E Succinate (VES) for 48 hours. The results showed that the expression of p16 p53 p21 and PCNA in SGC 7901 cells were significantly decreased after VES treatment with marked dose dependent response. The inhibition effect of VES to the p16 expression was the strongest. the less was p21 p53 and PCNA in turn. On the contrary, c fos protein expression was clearly induced by VES administration. Above results indicated that VES has potent anticarcinoma effect in Vitro.