用糖基化磷脂酰肌醇B7-1锚定肿瘤细胞膜进行免疫治疗

目的 研制抗肿瘤免疫治疗的新疫苗.方法 用蛋白转化法将B7-1锚定在肿瘤细胞膜上,免疫C57BL-6小鼠后,一组小鼠取脾细胞进行T细胞扩增和细胞毒T淋巴细胞(CTL)功能检测,另一组小鼠进行肿瘤细胞接种试验.结果 用GPI-B7-1修饰的肿瘤细胞膜免疫小鼠后诱发了肿瘤特异性T细胞扩增和CTL.且该疫苗有一定的保护小鼠免受肿瘤细胞侵袭的作用.结论 GPI蛋白转化法为人类抗肿瘤免疫治疗提供了新的、有效的修饰肿瘤细胞膜的方法.

Glycosyl-phosphatidylinositol-B7-1 anchors into tumor cell membranes: a novel approach to tumor immunotherapy

Objective To develop therapeutic cancer vaccines for use in human immunotherapy. Methods Stable incorporation of B7-1 on tumor cell membranes was achieved by incubating the isolated tumor membranes with purified GPI-B7-1. C57BL/6 mice were immunized with the modified tumor membranes, and then spleens of some mice were harvested for T cell proliferation and CTL assays. The other immunized mice were injected RM-1 cells for tumor challenge experiments. Results Immunization of C57BL/6 mice with the modified RM-1 tumor membranes induced tumor-specific T-cell proliferation and CTLs. In addition, a ceaain extent immunization with these RM-1 membranes could protect mice from parental tumor challenge. Conclusion Protein transfer of glycolipid-anchored molecules provides an novel and efficient approach to modify tumor membranes for human immunotherapy.