聚乳酸/壳聚糖纤维复合支架的制备与性能

摘要背景：聚乳酸/壳聚糖纤维复合支架材料既可提高支架的力学性能，又可中和聚乳酸的酸性降解产物，提高生物相容性，从而满足组织工程支架的要求。目的：制备用于组织工程的聚乳酸/壳聚糖纤维复合支架。方法：采用热致相分离法制备了聚乳酸/壳聚糖纤维复合支架。测定了复合支架的微观形貌、孔隙率、压缩模量、降解特性、蛋白质吸附特性。结果与结论：复合支架具有纳米微米共存的亚微观结构。在聚乳酸纳米纤维网络中引入壳聚糖纤维，有效地增强了复合支架的压缩模量和蛋白质吸附能力，复合支架压缩模量为纯聚乳酸纳米支架的3.75倍，蛋白质吸附能力比纯聚乳酸纳米支架提高了112%。体外降解实验表明复合支架降解液的pH值随时间的下降明显变缓。提示，在聚乳酸纳米纤维网络中引入壳聚糖纤维，可有效增强支架的压缩模量，提高蛋白质吸附能力，并可有效减缓聚乳酸降解过程中pH值的下降，克服酸性产物引发的无痛性炎症问题。关键词：聚乳酸；壳聚糖；纳米复合；增强；支架doi:10.3969/j.issn.1673-8225.2010.42.015

Preparation and characterization of poly-L-lactic acid/chitosan fibers composite scaffold

AbstractBACKGROUND: Poly-L-lactic acid (PLLA)/chitosan fiber composite scaffold could enhance the mechanical property of the scaffold, buffer the acid product of PLLA degradation, improve the biocompatibility, thus meeting the requirement of tissue engineering scaffolds. OBJECTIVE: To fabricate PLLA/chitosan fibers composite scaffold for tissue engineering.METHODS: The PLLA/chitosan fibers composite scaffold was prepared by thermally induced phase separation method. The micro-morphology, porosity, compressive modulus, degradation and protein adsorption of the scaffold were investigated. RESULTS AND CONCLUSION: The morphology of composite scaffolds showed a combined nanometer and micrometer structure. Adding chitosan fibers to PLLA network can enhance the compressive modulus and protein adsorption of PLLA/chitosan fiber composite scaffold, increased 275% and 112% compared with those of a pure PLLA scaffold. Experiment of the in vitro degradation demonstrated that pH value of composite scaffolds degradation solution declined more slowly with the time. The PLLA/chitosan fiber composite scaffold can enhance the compressive modulus and protein adsorption, also buffer the pH decline during PLLA degradation process, and effectively reduce the indolent inflammation induced by acid product.