地甘口服液对免疫介导再生障碍性贫血小鼠胸腺细胞增殖周期的影响

目的 :研究中药复方地甘口服液对免疫介导再生障碍性贫血小鼠模型胸腺淋巴细胞增殖周期的影响。方法 :选用Balb/c小鼠为实验对象 ,在接受亚致死剂量射线照射后 ,立即注射DBA/2小鼠胸腺、淋巴结混合细胞 ,形成免疫介导再障小鼠模型 ,分组处理后 ,摘取胸腺制成单细胞悬液 ,然后采用流氏细胞仪(FACS)进行分析。结果 :免疫介导再障小鼠胸腺细胞绝大部分停留在G0 /G1期 ,S期和G2 +M期细胞显著减少 ,与正常对照组比较有显著性差异 (P <0 0 1)。两种中药复方均能促进小鼠胸腺细胞的分化增殖 ,降低细胞的凋亡率 ,但地甘口服液的效果明显优于四物口服液 (P <0 0 5)。结论 :地甘口服液可明显促进免疫介导再障小鼠的胸腺淋巴细胞分化增殖 ,延缓免疫细胞的衰老凋亡 ,效果优于四物口服液组。

Effect of Digan Oral Liquid on Thymic Lymphocyte Proliferation Cycle in Immune Mediated Aplastic Anemia in Mice

To study the effect of Digan oral liquid (DGOL) on cell proliferation cycle of thymic lymphocyte in immune mediated aplastic anemia (IMAA) in mice. Methods: Balb/c mice were used in the experiment, after having exposed under a sublethal amount of radiation, the mice were received injection of mixed cells of thymus and lymphnode of DBA/2 mice to form the IMAA models. The model mice were grouped and treated, then their thymus was taken to prepare the single cell suspension for analysis by flow cytometry. Results: Majority of thymocyte of IMAA mice stopped at the G 0/G 1 phase, and the cells in S and G 2 +M phases were significantly less than those in the normal control group, P<0 01. Both DGOL and Siwu oral liquid could increase the differentiation and proliferation of thymocytes and reduce the apoptosis rate of them, but DGOL showed a better effect, P<0 05. Conclusion: DGOL could significantly promote the differentiation and proliferation of thymocytes and reduce the apoptosis rate of them in IMAA mice, the effect of its is superior to that of Siwu oral liquid.