| 丝胶对2型糖尿病大鼠睾丸生精细胞PCNA表达的影响 |
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| 引用本文： | 付文亮,和亚强,付秀美,马洪伟,钟美蓉,陈志宏.丝胶对2型糖尿病大鼠睾丸生精细胞PCNA表达的影响[J].承德医学院学报,2009,26(4):353-355. |
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| 作者姓名： | 付文亮 和亚强 付秀美 马洪伟 钟美蓉 陈志宏 |
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| 作者单位： | 承德医学院,河北承德,067000 |
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| 摘 要： | 目的:研究丝胶对2型糖尿病大鼠血清睾酮水平和睾丸生精细胞PCNA表达的影响.方法:40只SD大鼠随机分为4组,正常对照组、糖尿病模型组、丝胶治疗组和阳性对照组,每组10只.糖尿病模型组、丝胶治疗组和阳性对照组大鼠均采用链脲佐菌素(Streptozotocin,STZ)连续腹腔注射建立2型糖尿病大鼠模型.待模型成功建立后,模型组大鼠不再作任何处理;丝胶治疗组大鼠给予丝胶灌胃(2.4g/kg/d),阳性对照组大鼠给予二甲双胍(55.33mg/kg/d)灌胃,时间均为35天.采用ELISA方法检测各组大鼠血清睾酮水平、SP免疫组化法检测睾丸生精细胞增殖细胞核抗原(PCNA)的表达.结果:糖尿病模型大鼠血清睾酮水平、睾丸生精细胞PCNA的表达均明显低于正常对照大鼠(P<0.01).丝胶可明显提高糖尿病大鼠血清睾酮水平和睾丸生精细胞PCNA的表达(丝胶治疗组与模型组比较:P<0.01);且丝胶治疗组与阳性对照组比较无明显差别(P>0.05).结论:丝胶可显著提高2型糖尿病大鼠睾酮的合成及睾丸生精细胞PCNA的表达,明显改善2型糖尿病大鼠的生精功能,且作用与二甲双胍相当.
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| 关 键 词： | 丝胶 糖尿病 睾丸 睾酮 增殖细胞核抗原 |
EFFECTS OF SERICINE ON PCNA EXPRESSION OF SPERMATOGENIC CELLS IN TYPE 2 DIABETES MELLITUS RATS TESTIS |
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| FU Wen-liang,HE Ya-qiang,FU Xiu-mei,et al.EFFECTS OF SERICINE ON PCNA EXPRESSION OF SPERMATOGENIC CELLS IN TYPE 2 DIABETES MELLITUS RATS TESTIS[J].Journal of Chengde Medical College,2009,26(4):353-355. |
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| Authors: | FU Wen-liang HE Ya-qiang FU Xiu-mei |
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| Institution: | FU Wen-liang,HE Ya-qiang,FU Xiu-mei,et al (Chengde Medical College,Hebei Chengde 067000,China) |
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| Abstract: | Objective:To study the effects of sericine on testosterone level in serum and PCNA expression of spermatogenic cells in diabetic rats testis.Methods:40 SD rats were randomly divided into 4 groups (n=10): normal control group, diabetes model group, sericine treatment group and positive control group.The type 2 diabetes model rats were induced by continuous injection of Streptozotocin (STZ) into abdominal cavity.After successfuly establishing the diabetes animal model:The rats in diabetes model group were not... |
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| Keywords: | Sericine Diabetes mellius Testis Testosterone PCNA |
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