|
|||||
|
|
| 阿蒂莫耶番荔枝内酯诱导MCF—7／ADR细胞凋亡的研究 | |
| 引用本文： | 程冠军 潘启超. 阿蒂莫耶番荔枝内酯诱导MCF—7／ADR细胞凋亡的研究[J]. 癌症, 1998, 17(3): 161-163,I000 |
| 作者姓名： | 程冠军 潘启超 |
| 作者单位： | 中山医科大学肿瘤研究所 |
| 摘 要： | 目的：探讨阿蒂莫耶番荔枝内酯对有多药抗药性的阿霉素的人乳腺癌细胞ＭＣＦ－７／ＡＤＲ凋亡的诱导作用，揭示其抗癌机制。方法；用ＭＴＴ法观察药物对细胞的生长抑制作用，ＴＵＮＥＬ法，流式细胞仪及光镜，电镜等技术研究癌细胞凋亡。结果；药物对癌细胞有明显生长抑制作用，ＩＣ５０Ｏ １０．５ｍｇ／Ｌ；光１镜，电镜可见染色质浓缩，聚集核膜下，核碎裂等改变；流式细胞仪检测到凋亡峰；１０ｍｇ／Ｌ药物作用４８小时，ＴＵＮ |
| 关 键 词： | 细胞凋亡 乳腺癌 阿蒂莫耶 番荔枝内酯 细胞株 |
Induction of apoptosis by annonaceons acetogenins in human breast cancer cell line MCF-7/ADR |
|
| Cheng Guanjun Pan Qichao Fu Liwu,et al.. Induction of apoptosis by annonaceons acetogenins in human breast cancer cell line MCF-7/ADR[J]. Chinese journal of cancer, 1998, 17(3): 161-163,I000 | |
| Authors: | Cheng Guanjun Pan Qichao Fu Liwu et al. |
| Affiliation: | Cheng Guanjun Pan Qichao Fu Liwu,et al.Cancer Institute,Sun Yat-sen University of Medical Sciences,Guangzhan,510060 |
| Abstract: | Objective: To investigate the induction of apoptosis of human breast cancer cell line MCF-7/ADR by Annonaceons acetogenins (AA) in order to demonstrate the mechanism of its anticancer action. Methods: MTT method, TUNEL, flow cytometry (FCM), light microscopy and transmission electronmicroscope were used. Results: MCF-7/ADR treated with AA underwent cell proliferation inhibition, IC50 is 10.5 mg/L; cell shrinkage, condensation cell chromatin clinged to periphery of the nuclear and fragmentated can be detected by light microscopy and transmission electronmicroscope, apopttotic peaks were also detected on FCM, the TUNEL-positive cells were 57.3% after being treated with AA at 10 mg/L for 48 hours. Conclusion: AA can induce apoptosis of MCF-7/ADR cell line. |
| Keywords: | Apoptosis Breast cancer Annonaceous acetogenins Cell line |
| 本文献已被 CNKI 维普 等数据库收录！ | |