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| 尿中多环芳烃单羟基代谢产物的液相色谱-质谱测定方法研究 | |
| 引用本文： | 黄传峰,闫慧芳,潘祖飞,戴宇飞,牛勇,王玉江,郑玉新. 尿中多环芳烃单羟基代谢产物的液相色谱-质谱测定方法研究[J]. 卫生研究, 2010, 39(3) |
| 作者姓名： | 黄传峰 闫慧芳 潘祖飞 戴宇飞 牛勇 王玉江 郑玉新 |
| 作者单位： | 1. 中国疾病预防控制中心职业卫生与中毒控制所,北京,100050 2. 北京市丰台区疾病预防控制中心 |
| 基金项目： | 国家杰出青年基金项目(No.30625031); 卫生公益行业科研专项(No.200802020) |
| 摘 要： | 目的建立同时测定尿液中萘、菲和芘的单羟基代谢产物1-羟基萘、2-羟基萘、3-羟基菲和1-羟基芘等的液相色谱-质谱检测方法。方法尿液样品经酶水解后,利用液液萃取进行净化提取,吹氮定容后进液相色谱质谱仪进行分析,利用内标标准曲线法对目标化合物进行定量。结果利用内标法进行定量分析,提高了方法的重现性和精密度,4种目标化合物的回收率在89.7%～103.0%范围内,批内和批间精密度分别在1.0%～4.2%和1.8%～5.8%范围内;1-羟基萘、2-羟基萘和1-羟基芘的检出限为0.2μg/L,3-羟基菲的检出限为0.1μg/L。结论液相色谱-质谱法灵敏度高,重现性和回收率好,适用于正常人群和多环芳烃暴露人群尿中1-羟基萘、2-羟基萘、3-羟基菲和1-羟基芘等多环芳烃代谢产物的检测。 |
| 关 键 词： | 多环芳烃 尿液 基质效应 羟基萘 羟基菲 液相色谱质谱 |
Using HPLC-MS /MS method to analyze monohydroxy metabolites of PAHs in urine |
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| HUANG Chuanfeng,YAN Huifang,PAN Zufei,DAI Yufei,NIU Yong,WANG Yujiang,ZHENG Yuxin. Using HPLC-MS /MS method to analyze monohydroxy metabolites of PAHs in urine[J]. Journal of hygiene research, 2010, 39(3) | |
| Authors: | HUANG Chuanfeng YAN Huifang PAN Zufei DAI Yufei NIU Yong WANG Yujiang ZHENG Yuxin |
| Affiliation: | HUANG Chuanfeng,YAN Huifang,PAN Zufei,DAI Yufei,NIU Yong,WANG Yujiang,ZHENG Yuxin National Institute for Occupational Health , Poison Control,Chinese Center for Disease Control , Prevention,Beijing 100050,China |
| Abstract: | Objective To establish a HPLC-MS /MS method for the simultaneous determination of 4 monohydroxy metabolites of polycyclic aromatic hydrocarbons(PAHs) in urine,including 1-naphthol,2-naphthol,3-phenanthrol and 1-pyrenol).Methods After being treated by enzymatic hydrolysis,urine samples were purified by liquid-liquid extraction,evaporated under nitrogen to constant volume and examined by HPLC-MS /MS.Target compounds were quantified by internal standard calibration curve.Results The reproducibility and precisi... |
| Keywords: | polycyclic aromatic hydrocarbons urine matrix effects naphthol phenanthrol liquid chromatography-tandem mass |
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