远志皂苷对脂多糖诱导的帕金森病模型大鼠的作用及机制研究

目的 通过模拟中药口服、疗程用药的方式,探究远志皂苷(Tenuigenin,TEN)对细菌脂多糖(lipopolysaccharide, LPS)诱导的帕金森病(Parkinson's disease, PD)模型大鼠的作用及机制。方法 实验分5个组,通过动物行为学测试、大鼠黑质及血浆中炎性反应因子含量测定、黑质多巴胺(dopamine,DA)能神经元存活率检测和主要脏器组织形态学切片观察,研究TEN对炎症性PD模型大鼠的作用及机制。结果 TEN能够改善炎症性PD模型大鼠的行为学损伤,降低黑质DA能神经元的病死率,抑制黑质炎性反应因子含量,对主要脏器的组织形态学无影响。结论 TEN对PD模型大鼠具有神经保护作用,其机制与抗感染活性密切相关。

Effects and mechanism of Tenuigenin on lipopolysaccharide induced Parkinson's disease model

Objective To investigate the effects of Tenuigenin (TEN) on lipopolysaccharide (LPS) -induced rat model of Parkinson's disease (PD). Methods Five experimental groups were set up to analyze the effect and mechanism of TEN on PD rats, with animal behavior tests, detection of inflammatory cytokines in the substantia nigra and plasma, detection of the survival rate of dopamine (DA) neurons and observation of morphological sections of major metabolic organs. Results The treatment with TEN improved motor impairment induced by LPS. Also, TEN significantly ameliorated the degeneration of dopaminergic neurons and inhibited the secretion of proinflammatory cytokines in substantia nigra. Furthermore, TEN had no effect on the major metabolic organs. Conclusion TEN showed neuroprotective effect on PD rats. The mechanism is related to the anti-inflammatory activity of TEN.