CD248在肾透明细胞癌中的表达及临床意义CSCD

目的探讨内皮唾酸蛋白(CD248)在肾透明细胞癌(ccRCC)组织中的表达及临床意义。方法收集115例ccRCC组织及对应癌旁组织的石蜡标本,同时收集其中17例ccRCC组织及对应癌旁组织的新鲜标本。qRT-PCR检测17例ccRCC组织及对应癌旁组织中CD248 mRNA的表达;免疫组织化学法检测115例ccRCC患者石蜡标本的CD248蛋白水平,分析CD248表达与ccRCC临床病理指标及预后的相关性。结果ccRCC组织中的CD248阳性表达免疫组织化学反应强度显著强于癌旁组织,差异有统计学意义(P=0.0061),且CD248蛋白阳性信号定位于细胞核及细胞质。CD248 mRNA在癌旁组织中的表达水平明显低于ccRCC组织(P=0.0026)。CD248高表达组组间总生存率明显低于CD248低表达组(44.4 vs.57.2月,P=0.017)。结论CD248的表达可能参与ccRCC的发生发展。与癌旁组织比较,CD248在ccRCC组织中高表达;CD248高表达的ccRCC患者术后生存时间较短。

CD248 Expression in Clear Cell Renal Cell Carcinoma and Its Clinical Significance

Objective To investigate the expression of CD248 (endosialin) in clear cell renal cell carcinoma (ccRCC) tissues and its clinical significance. Methods We collected fresh specimens from 17 ccRCC tissues and adjacent tissues, as well as paraffin specimens from 115 ccRCC tissues and adjacent tissues. qRT-PCR was used to detect the expression of CD248 mRNA in 17 cases of ccRCC tissues and corresponding adjacent tissues. Immunohistochemistry was used to detect the level of CD248 protein in paraffin-embedded specimens of 115 ccRCC patients. The correlation of CD248 expression with clinicopathological indicators and prognosis of ccRCC patients were analyzed. Results Compared with adjacent noncancerous tissues, CD248 mRNA expression (P=0.0026) and the average tissue immune response score (P=0.0061) were significantly higher in ccRCC specimens. The positive signal of CD248 protein was localized in the cytoplasm and part of the nucleus. The overall survival (OS) of high CD248 expression group was significantly lower than that of low CD248 expression group (44.4 vs. 57.2 months, P=0.017). Conclusion CD248 expression may be involved in the development of ccRCC. Compared with adjacent tissues, CD248 is highly expressed in ccRCC tissues. ccRCC patients with high CD248 expression have shorter survival time.