首页 | 本学科首页   官方微博 | 高级检索  
     

MYEOV对人胰腺癌细胞增殖和迁移的影响
引用本文:吕根,严俊,任龙飞,周文策. MYEOV对人胰腺癌细胞增殖和迁移的影响[J]. 肿瘤防治研究, 2020, 47(5): 324-329. DOI: 10.3971/j.issn.1000-8578.2020.19.1387
作者姓名:吕根  严俊  任龙飞  周文策
作者单位:1. 730000 兰州,兰州大学第一临床医学院;2. 730000 兰州,兰州大学第一医院普外二科
基金项目:甘肃省科技厅重点研发基金(17YF1FA128),甘肃省卫生行业科研计划项目(GSWSKY2018-51)
摘    要:
目的探讨骨髓瘤过表达基因MYEOV在人胰腺癌细胞中的表达情况及其下调对胰腺癌SW1990细胞增殖和迁移能力的影响。方法构建慢病毒载体GV248,转染胰腺癌细胞株SW1990获得SW1990-sh1和SW1990-sh2两个实验组,以空白质粒转染作为阴性对照组,未干预细胞为空白对照组。qPCR和Western blot检测转染前后MYEOV mRNA与蛋白的表达水平;CCK-8法测定细胞增殖能力;划痕实验分析细胞迁移能力。qPCR检测TGF-β/SMAD通路中相关SMADs mRNA表达水平。结果与正常胰腺导管上皮细胞系HPDE6相比,MYEOV mRNA在6株胰腺癌细胞中表达水平明显增加(P<0.01);但仅在PANC-1和SW1990细胞系中检测到低水平MYEOV蛋白表达。实验组中MYEOV mRNA和蛋白表达均明显下调。与对照组相比,SW1990细胞的增殖和迁移能力明显下降(P<0.001)。下调MYEOV能降低TGF-β通路中SMAD1、SMAD4、SMAD5和SMAD9 mRNA表达(P<0.01),而SMAD2、SMAD3和SMAD7 mRNA仅在SW199...

关 键 词:胰腺癌  MYEOV  孤儿基因  TGF-β通路  增殖  迁移
收稿时间:2019-11-06

Effect of MYEOV on Proliferation and Migration of Human Pancreatic Cancer Cells
LYU Gen,YAN Jun,REN Longfei,ZHOU Wence. Effect of MYEOV on Proliferation and Migration of Human Pancreatic Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2020, 47(5): 324-329. DOI: 10.3971/j.issn.1000-8578.2020.19.1387
Authors:LYU Gen  YAN Jun  REN Longfei  ZHOU Wence
Affiliation:1. The First Clinical Medical College, Lanzhou University, Lanzhou 730000, China; 2. Second Department of General Surgery, The First Hospital of Lanzhou University, Lanzhou 730000, China
Abstract:
Objective To investigate the expression of MYEOV in human pancreatic cancer cells and its effect on the proliferation and migration of pancreatic cancer SW1990 cells after knockdown. Methods The lentiviral vector GV248 was constructed and infected SW1990 cells to obtain two experimental groups (SW1990-sh1 and SW1990-sh2), while the blank plasmid was transfected as a negative control group and the untreated cells were taken as blank control group. MYEOV mRNA and protein expression were detected by qPCR and Western blot before and after transfection. The cells proliferation was determined by CCK-8 assay; the cell migration ability was analyzed by scratch test; The mRNA expression of SMADs in the TGF-β/SMAD signaling pathway was detected by qPCR. Results Compared with the pancreatic ductal epithelium cell line HPDE6, the mRNA expression of MYEOV in six pancreatic cancer cells were significantly increased (P<0.01). However, low expression of MYEOV protein was detected only in PANC-1 and SW1990 cells. The mRNA and protein expression of MYEOV in the experimental groups were significantly downregulated. The proliferation and migration of SW1990 cells were lower than the control group (P<0.001). MYEOV knockdown could reduce the mRNA expression of SMAD1, SMAD4, SMAD5 and SMAD9 in TGF-β pathway, while the expression of SMAD2, SMAD3 and SMAD7 mRNA were only down-regulated in the SW1990-sh2 group (P<0.001). Conclusion MYEOV mRNA is overexpressed in pancreatic cancer cells. MYEOV could promote the proliferation and migration of pancreatic cancer cells via TGF-β/SMAD pathway.
Keywords:Pancreatic cancer   MYEOV   Orphan gene   TGF-β pathway   Proliferation   Migration  
本文献已被 维普 等数据库收录!
点击此处可从《肿瘤防治研究》浏览原始摘要信息
点击此处可从《肿瘤防治研究》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号