TRIM28在胃癌中表达的临床意义及对胃癌细胞侵袭和迁移的影响

目的 分析三结构域蛋白28（tripartite motif-containing protein 28，TRIM28）在胃癌中的表达及与胃癌患者临床预后的关系；探讨TRIM28对胃癌细胞侵袭迁移的影响及机制。方法 结合TCGA数据库和实时荧光定量PCR （quantitative real-time polymerase chain reaction，qRT-PCR），分析胃癌中TRIM28的表达。结合Kaplan-Meier Plotter，探究TRIM28与胃癌患者预后的关系。应用AGS胃癌细胞系，以siRNA干扰TRIM 28表达后，以Transwell和划痕愈合试验探究TRIM28对AGS侵袭和迁移的影响；并用Western blotting检测Wnt/β-catenin信号通路关键蛋白变化。结果 TRIM28在胃癌组织及细胞中异常高表达；且高表达的胃癌患者预后差。Transwell和划痕愈合试验结果显示，敲低TRIM28表达的AGS细胞侵袭和迁移能力均下降，差异具有统计学意义（P<0.01，P<0.05）。Western blotting检测结果显示，干扰TRIM28使β-catenin和c-Myc蛋白的表达明显下降。结论 TRIM28在胃癌中的表达增加且其表达与胃癌不良预后明显相关，TRIM28促进胃癌细胞的侵袭和迁移。

Clinical importance of TRIM28 expression in gastric cancer and its effect on invasion and migration of gastric cancer cells

Objective To investigate the expression of tripartite motif-containing protein 28 (TRIM28) in gastric cancer (GC) tissues and cells and the relationship with clinical prognosis as well as its effect on the invasion and migration properties of GC cells and the underlying mechanisms. Methods The TCGA database and the quantitative real-time polymerase chain reaction (qRT-PCR) were used to analyze the expression level of TRIM28 in GC tissues and cells. The Kaplan-Meier Plotter database was applied to explore the relationship between TRIM28 expression and the clinicopathological features of GC patients. AGS GC cell line was used in this study, and the cell function was explored after TRIM28 knockdown with siRNA. The effects of TRIM28 on the invasion and migration of AGS cells were determined with Transwell assay and wound healing assay, respectively. The expressions of key proteins in Wnt/β-catenin signaling pathway were detected by Western blotting. Results The expression level of TRIM28 in GC tissues and cells was significantly higher. The overall survival of patients with high expression of TRIM28 were significantly lower than those with low expression of TRIM28. The invasion property of AGS cells was impaired after TRIM28 knockdown compared to the control siRNA-transfected cells (P<0.01). The results of wound healing assay showed that the migration ability of AGS cells was impaired after TRIM28 knockdown compared to the control siRNA-transfected cells (P<0.05). The expressions of β-catenin and c-Myc proteins were down-regulated with TRIM28 knockdown. Conclusion The expression of TRIM28 was increased in GC specimens. It promoted the invasion and migration of GC cells, associated with poor prognosis of the cancer.