肺癌患者血清可溶性B7-H4分子的检测及其临床意义

目的:分析肺癌患者血清中可溶性分子B7-H4的水平变化并探讨其临床意义。方法:确诊为肺癌的患者37例,用ELISA夹心法检测患者治疗前后血清中的B7-H4水平变化,用微粒子化学发光法测定患者治疗前后肿瘤抗原125(CA125)水平的变化,分析比较B7-H4与CA125水平变化与患者病理类型及其诊断的相关性;选取30例健康人群血清作为对照。结果:与健康组相比:治疗前患者血清中B7-H4水平(1.53±0.22)μg/L明显高于治疗后的水平(0.96±0.17)μg/L,差异有统计学意义(P<0.05);同时也发现腺癌患者血清B7-H4水平(2.05±0.33)μg/L远高于小细胞癌、大细胞癌和鳞癌患者,差异有统计学意义(P<0.05)。B7-H4分子的敏感度为62.2%低于CA125分子的75.7%,而二者联合使用时的敏感度可达91.9%。肺癌患者血清B7-H4水平与CA125水平呈正相关(P<0.05)。结论:肺癌患者血清中B7-H4水平变化与病理类型密切相关,在腺癌的诊断上B7-H4分子可作为一个重要的参考指标,而在鳞癌的诊断上不建议采用该分子作为一个诊断指标。

Detection of Soluble B7-H4 Molecules in Serum of Lung Cancer Patients and Its Clinical Significance

Objective: To detect the level change of soluble B7-H4 in molecule serum of lung cancer patients and to discuss its clinical significance.Methods: ELISA sandwich method was used to detect the level of soluble B7-H4 in serum of 37 cases of lung cancer,and microparticle chemiluminescence method was used to detect the change of CA125 levels in patients before and after treatment.Then correlation of soluble B7-H4 to CA125 levels,pathological types and diagnosis were analyzed.The serum samples of 30 healthy women were selected as control.Results: Compared with the healthy group,the serum level of soluble B7-H4 in lung cancer patients before treatment was obviously higher than that after treatment([1.53±0.22] μg/L vs.[0.96±0.17] μg/L),with statistically significant difference(P<0.01).Also it were found that B7-H4 levels in serum of adenocarcinoma patients was(2.05 ± 0.33) μg/L,much higher than that in small cell carcinoma,large cell carcinoma and squamous cell carcinoma patients,and the difference was statistically significant(P<0.05).The sensitivity of B7-H4 molecules was 62.2%,lower than that of the CA125 molecule which was 75.7%;while the sensitivity became up to 91.9% when the two were used in combination.The serum level of sB7-H4 was positively correlated with that of CA125(P<0.01)in lung cancer patients.Conclusion: Serum levels of B7-H4 in patients with lung cancer is closely related to the pathological types.B7-H4 molecule can be used as an important reference index in diagnosis of adenocarcinoma,but was not recommended as an indicators in the diagnosis of squamous cell carcinoma.