前列腺素EP4受体激动剂HY-128686对实验性缺血性脑梗死的神经保护作用

目的:研究前列腺素EP4受体激动剂HY-128686对大鼠短暂性局灶性脑缺血的治疗作用及其机制.方法:采用线栓法制备大鼠大脑中动脉闭塞模型,使用不同剂量HY-128686或空白溶剂处理后,大鼠进行神经系统缺陷评估,收集脑样本,计算梗死面积,检测基质金属蛋白酶9(MMP-9)、白介素6(IL-6)、白介素1β(IL-1β...

Neuroprotective effect of prostaglandin EP4 receptor agonist HY-128686 on experimental cerebral ischemic stroke

Objective To study the protective effect and mechanism of prostaglandin EP4 receptor agonist HY-128686on transient focal cerebral ischemia in rats. Methods The rat middle cerebral artery occlusion model was preparedby the suture method. After treatment with different doses of HY-128686 or blank solvent， the rats were evaluated forneurological deficits， samples were collected， infarct size was calculated， and changes in the expression of matrixmetalloprotein 9（ MMP-9），interleukin 6（ IL-6）， interleukin 1β（ IL-1β） and vascular tight binding protein weredetected. Results HY-128686 treatment significantly reduced the rat cerebral infarction volume， reduced MMP-9，inhibited the expression of IL-1β， IL-6 and zonula occludens-1， and promoted the recovery of nerve function.Conclusion HY-128686 reduces the expression of pre-inflammatory factors including IL-1β， IL-6 and MMP-9 byactivating EP4. As a result， the degradation of tight junction protein is inhibited， the integrity of blood brain barrier ismaintained， and the infarct area to exert neuroprotective effects is reduced.