Rab1A对多发性骨髓瘤细胞系8226增殖和凋亡的影响

目的 探讨Rab1A对多发性骨髓瘤(MM)细胞系8226增殖和凋亡的影响.方法 采用siRNA干扰RabIA表达,将多发性骨髓瘤细胞8226分为空白对照组、阴性对照组和Rab1A siRNA组.其中空白对照组的多发性骨髓瘤细胞8226不做任何处理,阴性对照组的多发性骨髓瘤细胞8226转染阴性对照siRNA.Rab1A ...

Effects of Rab1A on proliferation and apoptosis of multiple myeloma cell line 8226

Objective To investigate the effect of Rab1A on proliferation and apoptosis of multiple myeloma(MM)cell line 8226.Methods The siRNA interference was used to knockdown Rab1A gene.The multiple myeloma cell line 8226 was divided into blank control group,negative control group and Rab1A siRNA group.In the blank control group,the multiple myeloma cells were not treated.Multiple myeloma cells 8226 in the negative control group were transfected with negative control siRNA.The Rab1A siRNA group was transfected with Rabl A-targeted siRNA.The effect of Rab1A on multiple myeloma cell 8226 proliferation was analyzed by colony forming test and cell counting kit-8(CCK-8)assay.The apoptosis of multiple myeloma cell 8226 was detected by flow cytometry.Western blotting and Real-time PCR were used to observe the effect of Rab1A siRNA on the expression of c-Myc,cyclin D1,Bcl-2 and Bax.Results The expressions of Rab1A mRNA and Rab1A protein in the Rab1A siRNA group were significantly down-regulated compared with those in the negative control group.The result of colony formation and CCK-8 assay showed that Rab1A siRNA inhibit the proliferation of multiple myeloma cells 8226.The early and late apoptosis ratio of multiple myeloma cell 8226 in Rab1A siRNA group increased significantly compared with the negative control group(P<0.05).The expression of cyclin D1 and Bcl-2 in the Rab1A siRNA group were significantly down-regulated compared with the negative control group(P<0.05),and the expression of c-Myc and Bax were significantly up-regulated compared with the negative control group(P<0.05).Conclusion Rab1A may promote the proliferation of multiple myeloma cells 8226 by regulating the expression of c-Myc,cyclin D1,Bcl-2 and Bax,while Rabl A siRNA can effectively inhibit the expression of Rabl A in rpmi-8226 cells,thereby inhibiting its proliferation and promoting apoptosis.