静电场调控p53合成对成纤维细胞增殖周期的影响*

目的：研究驻极体静电场调控p53 合成对成纤维细胞生长和增殖的影响。方法：将表面电位为0 的PP 膜设 为对照组，将表面电位为5 000 V 和-5 000 V 的驻极体分别设为5 000 V 驻极体组（5 000 V 组）和-5 000 V 驻极 体组（-5 000 V组）。采用常温等离子体放电系统将聚丙烯薄膜分别制成5 000 V驻极体和-5 000 V驻极体。将正 极性和负极性的驻极体分别作用于对数生长期的成纤维细胞48 h，借助于等效表面电位测量系统检测细胞生长环 境的静电场稳定性。通过CCK-8 实验研究正极性和负极性驻极体对成纤维细胞增殖能力的影响，并与对照组做 比较，通过流式细胞仪研究正极性和负极性驻极体对成纤维细胞生长周期的影响。通过实时荧光定量PCR研究在 静电场环境下生长的成纤维细胞中p53 mRNA的表达。结果：正极性和负极性的驻极体可提供稳定的静电场作用 于成纤维细胞。负极性驻极体使细胞快速通过G1期促进成纤维细胞的生长，而正极性驻极体通过阻断G1期抑制 成纤维细胞生长。经负极性驻极体作用，成纤维细胞中p53 mRNA的表达量较对照组明显减少；经正极性驻极体 作用，成纤维细胞中p53 mRNA 的表达量较对照组显著增加。结论：电场可引起成纤维细胞的细胞周期发生变化， 从而调控细胞的分化与增殖。负极性驻极体具有促进成纤维细胞的生长作用，正极性驻极体可抑制成纤维细胞生 长。驻极体产生的静电场通过调控细胞内p53 mRNA 的表达实现对成纤维细胞生长的调控。

Effect of regulating p53 synthesis by electrostatic field on proliferation cycle of fibroblast

Objective To study the effect of regulating p53 synthesis by electrostatic field on proliferation cycle of fibroblasts. Methods 5 000 V electret and -5 000 V electret of polypropylene film were prepared by room temperature plasma discharge system. The electrets with positive and negative polarity were applied to fibroblasts in logarithmic phase for 48 h， and the stability of electrostatic field was measured by equivalent surface potential. The effect of positive and negative electrets on fibroblast proliferation were studied by CCK-8 method and compared with control group. The effect of positive and negative electrets on fibroblast growth cycle were studied by flow cytometry. The expression of p53 mRNA in fibroblasts growing in the electrostatic field was studied by real-time fluorescent quantitative PCR. Results Positive/negative electrets could provide stable electrostatic field to the fibroblasts ； negative electrets promoted fibroblast growth through G1 phase， while positive electrets inhibited fibroblast growth by blocking G1 phase ； the expression of p53 mRNA in fibroblasts treated with negative polar electret was significantly lower than that in control group， and the expression of p53 mRNA in fibroblasts treated with positive polar electret was significantly higher than that in control group. Conclusion Negative electret can promote the growth of fibroblast while positive electret can inhibit the growth of fibroblasts. The electrostatic field produced by electret regulates the growth of fibroblast by regulating the expression of p53 mRNA.