| Survivin 基因在增殖性玻璃体视网膜病变增殖膜中的表达 |
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| 引用本文： | 王俊艳,颜华,王世忠,李金茹,刘皓.Survivin 基因在增殖性玻璃体视网膜病变增殖膜中的表达[J].河北医学,2006,12(9):898-900. |
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| 作者姓名： | 王俊艳 颜华 王世忠 李金茹 刘皓 |
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| 作者单位： | 1. 天津医科大学组织胚胎学教研室,天津,300070
2. 天津医科大学总医院眼科,天津,300000 |
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| 摘 要： | 目的:观察Survivin基因在增殖性玻璃体视网膜病变增殖膜中的表达。方法:取已确诊为PVR并行玻璃体切除术患者的PVR增殖膜,标本于术后立即由贮液瓶取至无菌器皿冰温运回,取出增殖膜放入多聚甲醛固定,并常规乙醇脱水,二甲苯透明、浸蜡、包埋、制备5μm连续切片,将切片行常规H-E染色,采用Survivin多克隆抗体(抗人及抗兔)行免疫组织化学实验,检测Survivin基因在玻璃体和增殖膜中的表达。结果:Survivin基因在PVR增殖膜中呈阳性表达,且随着PVR临床分级的增高,免疫组织化学染色阳性细胞百分率逐渐升高。结论:认为PVR的形成是由于Survivin基因的异常表达促进了细胞增殖,阐明PVR的形成机制。
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| 关 键 词： | Survivin基因 增殖性玻璃体视网膜病变 增殖膜 |
| 文章编号： | 1006-6233（2006）09-0898-03 |
Expression of Survivin Gene in Periretinal Membranes of Proliferative Vitreoretinopathy |
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| WANG Jun -Yan, et al.Expression of Survivin Gene in Periretinal Membranes of Proliferative Vitreoretinopathy[J].Hebei Medicine,2006,12(9):898-900. |
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| Authors: | WANG Jun -Yan |
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| Institution: | Tianjin Medical University, Tianjin 300070, China |
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| Abstract: | Objective: To study the expression of survivin gene in periretinal membrane of prliferative vitreoretinopathy(PVR).Method: 14 periretinal membranes obtained from 14 cases of PVR with retinal detachment undergoing vitretomy were used to examine the expression of surviving gene by SABC method.Result: Survivin gene was expressed in 8 of the 14 cases.The reactions of surviving were observed in the cytoplasm and extracellular matrix.Conclusion: The abnormal expression of surviving gene inhibits apoptosis and it may participate in the progression of proliferative vitreoretinopathy. |
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| Keywords: | Survivin Proliferative Vitreoretinopathy |
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