用冻干致敏血球间接血凝法诊断伤寒的研究

作者应用间接血凝试验诊断伤寒,经过3年多的调查研究,证实该法具有微量、快速、敏感性高、特异性强等优点,并有早期诊断价值。检测121例伤寒患者血清,阳性检出率高达98.35%(119/121),而肥达氏反应阳性检出率仅为79.34%(TO)。血球致敏冻干后,冰箱保存有效期可达一年以上,携带外出和邮寄,均无需冷藏,为推广应用提供了方便条件。

Studies of Lyophilized Red Blood Cells in Passive Hemagglutination Test in Lab. Diagnosis of Typhoid Fever

we sensitized human O red blood cell by an extract antigen O from Sal. typhi strain O₀₀₁. The sensitized RBC was then freezedried in protective medium. A survey of 181 healthy normal persons by indirect hemagglution for typhoid O antibody showed a result presenting 114 negative and the remainder 1:5~1:40. Therefore, the titre 1:80 is denoted to be a starting point of positive.
Examination of 26 soldiers in a vaccine process revealed only 4 (1:5) positive before vaccination and all became positive 1 month after vaccination. The serum titre reduced 2 months later and returned to normal in 3 months.
121 cases of typhoid fever were studied and their serum hemagglutination test showed a high percentage of positive, 98.35% (119/121eases). Meanwhile the widel test showed 79.34% positive (T.O.).
The indirect hemagglutination test had been put into practice for 3 years. It has the advantages such as simple, rapid, minute amount, sensitive, specific and good for early diagnosis. An expiration date is provided for the lyophylized RBC as one year in cold.