| 吴茱萸RAPD体系构建及道地性遗传背景研究 |
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| 引用本文： | 曹亮,李顺祥,魏宝阳,黄丹,徐菲,佟志远.吴茱萸RAPD体系构建及道地性遗传背景研究[J].医学教育探索,2010,41(6):975-978. |
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| 作者姓名： | 曹亮 李顺祥 魏宝阳 黄丹 徐菲 佟志远 |
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| 作者单位： | 湖南中医药大学药学院 中药现代化重点实验室，湖南 长沙410208;1.湖南中医药大学药学院 中药现代化重点实验室，湖南 长沙410208； 2.湖南中医药研究院中药研究所，湖南 长沙410013;湖南农业大学生物科学技术学院，湖南 长沙410128;湖南中医药大学药学院 中药现代化重点实验室，湖南 长沙410208;湖南中医药大学药学院 中药现代化重点实验室，湖南 长沙410208;湖南中医药大学药学院 中药现代化重点实验室，湖南 长沙410208 |
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| 基金项目： | 国家“十一五”科技支撑计划课题(2006BAI09B02)；国家自然科学基金资助项目(30973877)；湖南省科学技术厅科技项目(2008SK3087) |
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| 摘 要： | Objective To establish molecular marker method of RAPD and to probe into the genetic background of Fructus Evodia .Methods Genomic DNA of Fructus Evodia was extracted by the DNA extraction kit method and then chose the RAPD primer; RAPD Analysis on 48 individuals of Fructus Evodia from three provinces have been done. The NTSYS-PC software was used to do cluster analysis. ResultsRAPD Analysis method was optimized for Fructus Evodia. RAPD Cluster analysis revealed that variety in different kinds of Fructus Evodia was significant Evodia rutaecarpa from Liuyang and E rutaecarpa var officinalis from other places gathered in two populations at the similarity coefficient of 0.68.In the similarity coefficient of 0.78, the samples fromTongren, Liuyang, Jiande, and Wenzhou gathered in clusters, the same as their region ethnic groups, respectively.Conclusion Genetic background of Fructus Evodia from varies of regions is obviously different and RAPD analysis is a good manner to probe geo authentic crude drug Fructus Evodia.
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| 关 键 词： | 吴茱萸药材 RAPD DNA 道地药材 |
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