动物及其产品中空肠弯曲菌PCR检测方法的建立

目的建立快速检测空肠弯曲菌的PCR方法。方法从鸡猪样品中分离培养获得空肠弯曲菌,以该菌特异的VS1基因保守序列为模板自行设计引物,扩增产物为516bp片段,同时进行特异性、灵敏度试验。结果显示该方法只对空肠弯曲菌有扩增产物出现,而其它细菌如大肠杆菌、沙门氏菌、无乳链球菌、粪链球菌、金黄色葡萄球菌、金黄色葡萄球菌ATCC、腐生葡萄球菌等均不能扩增出特异性片段,检测灵敏度达6 CFU/ml。结论该方法具有灵敏、高效、特异、稳定的特点。

PCR assay for the detection of Campylobacter jejuni in animal organs and animal products

The PCR assay was established for the rapid detection of Campylobacter jejuni in animal organs and animal products,in which a pair of primers was designed according to the species-specific conserved sequence of C. jejuni VSlgene and used as template in PCR assay. The amplified product was a fragment with 516 bps in length. Simultaneously, testings in specificity and sensitivity were also performed as usual. It was found that the amplified product occurred only for C. jejuni as demonstrated by this method of assay, and no amplified product could be demonstrated to other microorganisms, such as E. coli, Salmonella, Streptococcus agalactiae , S. f acalis, Staphylococcus aureus ATCC and saprophytic staphylococci . The detection sensitivity appeared to be 6 cfu/ml. It is evident that this method of assay is sensitive,specific,efficient and quite stable.