大网膜移植抑制大鼠脊髓损伤后神经细胞凋亡的实验研究

目的 探讨脊髓损伤（SCI)后神经细胞凋亡的发生机制和伤后立即行大网膜脊髓移植是否对神经细胞凋亡具有抑制作用。方法 制作程控电磁吸铁棒下落打击脊髓损伤模型，运用末端脱氧核苷酸转移酶介导生物素标记（TUNEL）方法检测细胞凋亡，分别观察SCI后1d、2d、7d组与伤后即刻施行大网膜移植组（存活7d）的细胞凋亡。结果 脊髓灰质区在伤后1d、、2d时TUNEL阳性细胞数最多；脊髓白质区在伤后1d时TUNEL阳性细胞数最多，伤后7d出现第2次高峰；伤后大网膜脊髓移植的TUNEL阳性细胞数较7d明显降低。结论 SCI后白质区和灰质区存在不同演变过程的神经细胞凋亡，SCI后即刻大网膜脊髓移植对细胞凋亡有抑制作用。

The effect of omentum transplantation on neurocytic apoptosis in the injured spinal cord of rat

Objective To explore the mechanism of neurocytic apoptosis after spinal cord injury in rats and the effect of instant administration of omentum transplantation.?Methods Rat model of spinal cord injury was established. Neurocytic apoptosis in the injured area was examined by using terminal deoxynucleotidyl mediated nick-end labeling (TUNEL). The alterations of TUNEL positive cells were examined consecutively in different groups of rats to reveal the effects of instant omentum transplantation after 7 d.?Results The number of TUNEL-positive cells in gray matter reached a maximum on the first 2 d after injury. In white matter, the number of TUNEL-positive cells showed a peak on 1d and a second peak on 7d. The numbers of TUNEL-positive cells were markedly reduced 7 d after omentum transplantation.?Conclusion Neurocytic apoptosis appears in the spinal cord after injury. Its characteristics in the gray matter are different from those in the white matter. Instant application of omentum transplantation can significantly inhibit the neurocytic apoptosis after spinal cord injury.