氧化型胆固醇诱导兔血管平滑肌细胞凋亡

目的：研究氧化型胆固醇(Triol与25-OH)对兔主动脉血管平滑肌细胞(vascular smooth muscle cell,VSMC)凋亡的诱导,并观察其时效与量效关系。方法：原代培养兔VSMC,利用光镜、电镜、DNA电泳及TUNEL法作为凋亡检测法。结果：光镜、电镜下可见典型的细胞凋亡形态学改变,电泳示“DNA ladder”,TUNEL法示正常兔VSMC凋亡率为3.62％,用Triol和25-OH不同质量浓度(5、10、15、20 mg*L－1)分别处理细胞24 h后,凋亡率明显升高;用Triol和25-OH（15 mg*L－1）分别处理细胞0、12、24、36 h后,凋亡率随时间延长而增加。结论：氧化型胆固醇(Triol与25-OH)可以诱导血管平滑肌细胞发生凋亡,且呈时间和剂量依赖性。

Oxysterols induced apoptosis in rabbit vascular smooth muscle cells

Objective：To investigate the apoptosis of rabbit vascular smooth muscle cells (VSMC) induced by oxysterols and observe the time and dose effect. Methods： Light miroscope, electron microscope, DNA agarose gel electrophoresis and TUNEL were uesed to detect the apoptosis of rabbit aortic VSMC. Results：The characteristic morphological features of apoptosis were observed under light and electron microscope; DNA electrophoresis showed “DNA Ladder”; TUNEL showed the apoptotic rate of normal rabbit VSMC was 3.62%. While treated with either Triol or 25-OH by different dose (5, 10, 15, 20 mg*L－1) and at different times (0,　12,　24,　36 h), the apoptotic rate increased significantly. Conclusion： Both Triol and 25-OH can induce apoptosis of vascular smooth muscle cells in a dose and time dependent manner.