Evaluation of a Rapid Detection Influenza Virus A Antigens Kit Using Paired Serum Antibody Test |
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| Authors: | Hangwei Chen Huimin Wang Xuehui Li Lanhua You Juan Wei Weixian Shi |
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| Affiliation: | 1.Department of Respiratory Medicine, The Military General Hospital of Beijing PLA (The Chinese People''s Liberation Army, PLA), Beijing, China.;2.Department of Respiratory Medicine, Beijing Pinggu Hospital, Beijing, China.;3.Beijing Centers for Diseases Control and Prevention (CDC) & Centers for Preventive Medical Research, Beijing, China. |
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| Abstract: | PurposeTo evaluate the feasibility for gold immunochromatographic assay (GICA) in rapid detection of influenza virus A infection.Materials and MethodsSeventy-three patients were enrolled. All patients contributed nasopharyngeal secretions and paired serum samples. Nasopharyngeal secretions was used for colloidal gold immunochromatographic rapid assay for influenza A virus immediately after the collection of specimen. Paired serum samples were used for the hemagglutination inhibition assay at the Centers for Disease Control and Prevention influenza network laboratory in Beijing.ResultsCompare GICA test to hemagglutination inhibition (HI) assay, the Kappa value was 0.402 and the p value in the paired χ2 test was higher than 0.05. Therefore, the difference was not statistically significant. The sensitivity of GICA was 50.0% and the specificity was 90.2%, and the negative predictive value was 90.2%.ConclusionThe sensitivity for Influenza A antigen detection by using GICA is relatively low, the specificity is relatively satisfactory. |
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| Keywords: | Influenza A virus colloidal gold immunochromatographic assay antibody rapid test |
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