| 河北省不同途径感染人群HIV-1基因亚型分析 |
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| 引用本文： | 赵翠英,李巧敏,李保军,赵宏儒,路新利,李岩,王莹莹,邢辉,何翔. 河北省不同途径感染人群HIV-1基因亚型分析[J]. 中国艾滋病性病, 2011, 0(4): 399-401 |
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| 作者姓名： | 赵翠英 李巧敏 李保军 赵宏儒 路新利 李岩 王莹莹 邢辉 何翔 |
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| 作者单位： | 河北省疾病预防控制中心;中国疾病预防控制中心性病艾滋病预防控制中心; |
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| 基金项目： | 河北省科学技术研究与发展计划(09276102D-2)~~ |
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| 摘 要： | 目的了解河北省不同途径感染人群艾滋病病毒Ⅰ型(HIV-1)亚型分布特点。方法从感染者的血浆样品中提取病毒RNA,反转录后采用套式聚合酶链反应(Nested PCR)扩增HIV-1 gag和env基因的部分片段,对PCR产物直接进行核苷酸序列测定,所获序列与各亚型国际参考株序列比对,进行系统进化树分析,确定基因型。结果对154份HIV-1感染者的样品进行扩增,得到了146份样品的HIV-1基因片段。发现6种HIV-1亚型和重组型,其中B’亚型61例(41.8%),CRF01_AE 59例(40.4%),CRF07_BC 16例(11.0%),CRF08_BC 6例(4.1%),C亚型和B01重组株各2例(1.4%)。血液途径传播为B’亚型,男男性行为传播以CRF01_AE和B’亚型为主。结论河北省不同途径感染人群HIV-1亚型分布具有明显的人群特征。血液途径传播者为单一B’亚型,性传播人群中亚型分布较广。
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| 关 键 词： | 艾滋病病毒Ⅰ型 感染途径 基因亚型 |
Subtype analysis of the gag and env genes for HIV-1 strains isolated from different population groups in Hebei province |
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| ZHAO Cui-ying,LI Qiao-min,LI Bao-jun,et al.. Subtype analysis of the gag and env genes for HIV-1 strains isolated from different population groups in Hebei province[J]. Chinese JOurnal of Aids & STD, 2011, 0(4): 399-401 |
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| Authors: | ZHAO Cui-ying LI Qiao-min LI Bao-jun et al. |
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| Affiliation: | ZHAO Cui-ying,LI Qiao-min,LI Bao-jun,et al.(Institute of AIDS/STD Control and Prevention,Hebei Provincial Centre for Disease Prevention and Control,Shijiazhuang,Hebei 050021,China) |
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| Abstract: | Objective To study the subtypes distribution of HIV-1 strains prevalent among different population groups in Hebei province.Method Viral RNA was extracted from plasma samples of the infected individuals,HIV-1 genes(env and gag) were amplified by RT and nested-PCR using specific primer pairs and sequenced directly.The acquired sequences were compared with international subtypes references and their phylogenetic trees were analyzed to determine the subtype.Results Among 154 HIV-1 antibody positive cases,146 H... |
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| Keywords: | Human immunodeficiency virus type l Route of infection Genetic subtype |
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