| 用双重式PCR检测缓慢细蚤体鼠疫菌 |
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| 引用本文： | 张洪英,吴明寿,郭英,张丽云,董兴齐.用双重式PCR检测缓慢细蚤体鼠疫菌[J].现代医药卫生,2002,18(10):859-859. |
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| 作者姓名： | 张洪英 吴明寿 郭英 张丽云 董兴齐 |
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| 作者单位： | 云南省大理地方病防治所,671000 |
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| 基金项目： | 云南省自然科学基金资助项目 (98C110M ) |
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| 摘 要： | 目的：探讨双重式PCR在检测缓慢细蚤带鼠疫菌的应用。方法：应用双重式聚合酶链反应（Pla-FI-PCR)技术，对感染鼠疫菌的30匹缓慢细蚤进行检验。结果：阳性29匹，阳性率为96．67％。结论：双重式聚合酶链反应适用于缓慢细蚤染带鼠疫菌的检测。
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| 关 键 词： | 双重式PCR 检测 缓慢细蚤体 鼠疫菌 |
| 文章编号： | 1009-5519(2002)10-0859-01 |
Detection of the Yersinia pestis in Lptopsylla segnis by the double primers polymerase chain reaction. |
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| Zhang Honging,Wu Mingshou,Guo Ying,et al..Detection of the Yersinia pestis in Lptopsylla segnis by the double primers polymerase chain reaction.[J].Modern Medicine Health,2002,18(10):859-859. |
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| Authors: | Zhang Honging Wu Mingshou Guo Ying |
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| Institution: | Zhang Honging,Wu Mingshou,Guo Ying,et al.Yunnan Institute of Endemic Diseases Prevention and Treatment,Dali yunnan 671000 |
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| Abstract: | Objecttive:To study the application of the double primers polymerase chain reaction(Pla-FI-PCR) for detecting the Yersinia pestis in Lptopsylla segnis.Methods:30 samples of Lptosylla segnis of infected plague was detected by the double primers(Pla-FI)PCR.Results:Of 30 samples of Lptopsylla segnis of infected plague,29 were positive,the positive rate was 96.6%.Conclusion:The double primers(Pla-FI) PCR can be applied for detecting the Yersinia pestis in Lptopsylla segnis. |
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| Keywords: | Lptopsylla segnis Yersinia pestis Double primers(Pla-FI)PCR |
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