丙戊酸钠对骨髓增生异常综合征细胞株SKM-1作用及其机制的研究

本研究探讨丙戊酸钠（sodium volproate,VPA）对骨髓增生异常综合征细胞SKM-1的作用及其机制。采用四甲基偶氮唑蓝（MTT）比色法检测VPA对细胞生长的抑制作用;采用流式细胞术检测不同浓度VPA作用后细胞凋亡的比例;采用RT-PCR技术检测c-flipl、c-flips及dlk1 mRNA表达的变化。结果发现,VPA对于SKM-1细胞生长具有抑制作用,且随时间的延长及浓度增高而增强;流式细胞术检测显示,细胞凋亡率随着VPA浓度的增加而增加;VPA可使SKM-1细胞中c-flipl、c-flips及dlk1mRNA表达明显降低,且随着VPA浓度增加而加重。结论：VPA可以通过促进细胞凋亡抑制SKM-1细胞增殖,c-flipl、c-flips及dlk1基因表达的改变可能在这一过程中发挥了重要作用。

Effect of Sodium Valproate on Human Meylodysplastic Syndrome Cell Line SKM-1 and Its Mechanism

This study was aimed to investigate the effect of sodium valproate（VPA） on human myelodysplastic syndrome cell line SKM-1 and its mechanism. The cell proliferation was determined by MTT assay, cell apoptosis was analyzed by flow cytometry. The expressions of c-flpl、c-flips and dlk1 mRNA were detected by RT-PCR. The results showed that VPA could inhibit the growth of SKM-1 cells in dose-and time-dependent manners. The flow cytometric analysis indicated that VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner. The expressions of c-flipl, c-flips and dlk1 mRNA in SKM-1 cell treated with VPA decreased using of VPA. It is concluded that VPA can induce apoptosis and inhibite proliferation of SKM-1 cells. In this process, the decreasing of c-flipl, c-flips and dlk1 mRNA expression may play important roles.