原发性血小板减少性紫癜患者T细胞受体Vβ亚家族基因的表达特点

目的：了解原发性血小板减少性紫癜（ITP）患者T细胞受体 Vβ亚家族基因表达特点。 方法： 采用反转录酶－多聚酶链反应（RT-PCR）方法检测5例ITP患者外周血T细胞TCR Vβ 24个亚家族基因表达情况，10例正常人作为对照。 结果： 正常人外周血T细胞表达全部24个TCR Vβ亚家族，而5例ITP患者外周血T细胞仅表达4-11个Vβ亚家族，主要为Vβ2（100％）和Vβ3（100％），其次为Vβ19（80％）和Vβ21（80％）部分。全部样本未检测出Vβ4、Vβ6、Vβ17、Vβ20、Vβ24亚家族表达。 结论： ITP患者外周血T细胞TCR Vβ基因谱系呈限制性表达，与其存在细胞免疫功能异常有关。

Expression of TCR Vβ subfamily in patients with idiopathic thrombocytopenic purpura

AIM: To investigate the expression of TCR Vβ subfamily in T cells in patients with idiopathic thrombocytopenic purpura (ITP). METHODS: The TCR Vβ 24 subfamily genes were amplified in peripheral blood mononuclear cells from 5 cases with ITP using RT-PCR, to observe the usage of TCR Vβ repertoire. 10 normal individuals served as controls. RESULTS: Only 4-11 Vβ subfamily in T cells were identified in ITP cases. The frequent expressions of Vβ subfamilies were Vβ2 (100%), Vβ3 (100%), Vβ19 (80%) and Vβ21 (80%), while the expression of Vβ4, Vβ6-12, Vβ17, Vβ20 and Vβ24 were not detected. All 24 Vβ subfamilies were detected in samples from normal individuals. CONCLUSION: The restricted expression of TCR Vβ subfamilies is one of the T-cell immune features in patients with ITP, indicating that it may be related to the disorder of cellular immune function in ITP.