丹参对去卵巢大鼠体质量、血脂和丙二醛的影响

背景:丹参已被广泛用于治疗心绞痛、缺血性脑卒中等缺血性心血管疾病,但丹参对去卵巢大鼠的作用尚须进一步明确。目的:观察口服丹参水煎剂对去卵巢大鼠体质量、进食量、血脂及丙二醛的影响。设计:完全随机分组设计,对照实验。单位:兰州大学基础医学院生理学与心理学研究所。材料:实验于2005-11/2006-12在甘肃省新药临床前研究重点实验室和兰州大学基础医学院生理学和心理学研究所实验室完成。选用健康雌性SD大鼠24只,3月龄,体质量(220±2)g。丹参水煎剂由甘肃省药检所鉴定并提取,相当于每毫升含生药1g。丙二醛试剂盒购于南京建成生物工程研究所。方法:①实验干预:将SD大鼠按随机抽签法分为3组,每组8只:假手术组,去卵巢组和丹参组。去卵巢组及丹参组大鼠行双侧去卵巢术,假手术组进行同样的手术过程,双侧切除与卵巢等大的脂肪,但保留卵巢。假手术组和去卵巢组大鼠术后自由饮水,丹参组大鼠术后自然喂饲1%浓度的丹参水煎剂,到第8天浓度逐渐增至12%,持续到实验结束,共55d。②实验评估:每天称各组大鼠的摄食量,每5d称各组大鼠的体质量。实验结束时,股动脉取测定血清中血脂水平,并按丙二醛试剂盒说明测定其血清水平。主要观察指标:各组大鼠体质量、摄食量、血脂水平、血清丙二醛水平。结果:大鼠24只全部进入结果分析。①体质量:手术前3组大鼠体质量相近(P>0.05)。术后10,20,25,55d去卵巢组大鼠体质量明显高于假手术组(P<0.01),术后20,25,55d丹参组明显低于去卵巢组(P<0.01)。②摄食量:去卵巢组大鼠术后15,40,55d摄食量均大于假手术组(P<0.05～0.01),丹参组大鼠3个时间点摄食量均小于去卵巢组(P<0.05～0.01)。③血脂、丙二醛水平:实验结束时,去卵巢组大鼠血清总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、三酰甘油水平均高于假手术组(P<0.05～0.01),丹参组三酰甘油和丙二醛水平明显低于去卵巢组(P<0.01,0.05)。结论:丹参能明显降低去卵巢大鼠的体质量,降低三酰甘油和丙二醛水平。

Effects of Salvia miltiorrhiza on body mass and levels of blood lipids and malondialdehyde in ovariectomized obese rats

BACKGROUND: Salvia miltiorrhiza is widely used to treat angina cordis, ischemic stroke and other ischemic cardiovascular diseases. However, the effects of Salvia miltiorrhiza on ovariectomized rats remain unclear.OBJECTIVE: To observe the effects of Salvia miltiorrhiza on the body mass, food intake, and levels of blood lipids and malondialdehyde (MDA) in ovariectomized rats.DESIGN: A completely randomized and controlled experiment.SETTING: Institute of Physiology and Psychology, School of Basic Medical Sciences, Lanzhou University.MATERIALS: The experiment was performed in the Key Laboratory of Pre-clinical Study for New Drugs of Gansu Province and the laboratory of Institute of Physiology and Psychology, School of Basic Medical Sciences, Lanzhou University from November 2005 to December 2006. Twenty-four healthy female SD rats of 3 months old and (220±2) g were selected. Salvia miltiorrhiza water decoction (equal to 1 g/mL crude drug) was identified and extracted by Drug Control Institute of Gansu Province; MDA kit was purchased from Nanjing Jiancheng Institute of Bioengineering.METHODS: ①The rats were randomly divided into three groups with 8 rats in each group: sham-operated group,ovariectomized group and Salvia miltiorrhiza group. The rats were underwent a bilateral ovariectomy except those in the sham-operated group, which were subjected to a removal of bilateral fat as much as ovariectomized group with the ovaries remained. Rats in sham-operated group and ovariectomized group freely drank water; rats in Salvia miltiorrhiza group freely took 1% water extracts from Salvia miltiorrhiza postoperatively, and the concentration of Salvia miltiorrhiza gradually increased to 12% on the eighth day, which was lasted until the end of the experiment (55 days). ②The food intake of rats in each group was monitored daily, and the body mass was measured every five days. At the end of the experiment, femoral artery blood samples of rats were collected to determine the levels of blood lipids. At the same time,MDA was measured according to the kit.MAIN OUTCOME MEASURES: The body mass, food intake, levels of blood lipids and malondialdehyde in each group.RESULTS: Twenty-four rats all entered the result analysis. ①The body mass of rats in 3 groups was nearly the same before operation (P ＞ 0.05). While the body mass in ovariectomized group on the postoperatively 10th, 20th, 25th, and 55th days was significantly higher than those in sham-operated group (P ＜ 0.01). The body mass in Salvia miltiorrhiza group on the postoperatively 20th, 25th, and 55th days was significantly lower than those in ovariectomized group (P ＜0.05-0.01). ②The food intake in ovariectomized group on the postoperatively 15th, 40th, and 55th days was significantly higher than those in sham-operated group (P ＜ 0.05-0.01), and that in Salvia miltiorrhiza group was significantly lower than those in ovariectomized group at those 3 time points (P ＜ 0.05-0.01). ③At the end of the experiment, the levels of total cholesterol, low density lipoprotein cholesterol, high density lipoprotein cholesterol and triglyceride in ovariectomized group were significantly higher than those in sham-operated group (P ＜ 0.05-0.01). The levels of triglyceride and MDA in Salvia miltiorrhiza group were significantly lower than those in ovariectomized group (P＜0.01, 0.05).CONCLUSION: Salvia miltiorrhiza can significantly reduce the body mass and levels of triglyceride and MDA in ovariectomized rats.