The measurement of serum cholinesterase activities by an integration strategy with expanded linear ranges and negligible substrate-activation期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

The measurement of serum cholinesterase activities by an integration strategy with expanded linear ranges and negligible substrate-activation

Authors:	Fei Liao Diyuan Yang Xiaolan Yang Yunsheng Zhao Hong Liao

Affiliation:	^a Chongqing Key Laboratory of Biochemical and Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University, Chongqing 400016, China ^b Key Laboratory of Laboratory Medical Diagnostics of the Ministry of Education, Chongqing Medical University, Chongqing 400016, China

Abstract:	Objectives To measure serum cholinesterase (SCHE) with an integration strategy. Design and methods At 54.0 μmol/L butyrylthiolcholine, SCHE initial rates were calculated with 50.0 μmol/L butyrylthiolcholine and maximal rates via an improved integrated method if substrate consumptions within 5.0 min were over 60%, or were determined by the classical initial rate method. Results The linear range was from 16 to 1560 nkat/L, and SCHE in clinic sera showed negligible substrate-activation. Conclusion This strategy was effective.

Keywords:	SCHE, serum cholinesterase BTCH, butyrylthiocholine S₀, initial substrate concentration K_m, Michaelis-Menten constant PSC, preset substrate concentration V_m, maximal reaction rate
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