| A Novel Anti-Human Syndecan-1（CD138） Monoclonal Antibody 4B3： Characterization and Application |
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| 引用本文： | Sun WP,Wang FM,Xie F,Wang GQ,Sun J,Yu GH,Qiu YH,Zhang XG. A Novel Anti-Human Syndecan-1（CD138） Monoclonal Antibody 4B3： Characterization and Application[J]. Cellular & molecular immunology, 2007, 4(3): 209-214 |
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| 作者姓名： | Sun WP Wang FM Xie F Wang GQ Sun J Yu GH Qiu YH Zhang XG |
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| 作者单位： | [1]Institute of Biotechnology and Clinical Immunology, Research Laboratory of Jiangsu Province, Soochow University, Suzhou 215007, China; [2]Physiology Department of Basic Medical School, Soochow University, Suzhou 215007, China; [3]Stem Cell Research Laboratory of Jiangsu Province, Soochow University, Suzhou 215007, China; |
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| 摘 要： | Syndecan-1 （CD138）, a member of integral membrane heparin sulfate proteoglycans, is an essential matrix receptor for maintaining the normal morphological phenotypes. In this study, we generated a specific mouse anti-human syndecan-1 monoclonal antibody （mAb） 4B3 and identified it by competition assay with the available syndecan-1 mAb （BB4）. Stained by 4B3, the expression of syndecan-1 was detected on tumor cell lines, such as 8226, U266, XG-1, XG-2, Daudi and Jurkat. The expression was also found on neuron stem cells. It was established that 4B3 mAb could inhibit XG-1 and XG-2 proliferation. The data not only determined that 4B3 mAb was a functional anti-human syndecan-1 mAb, but also indicated that syndecan-1 might be a valuable surface antigen and play an important role in regulation of tumor pathology and differentiation of neural stem cells. This novel antibody 4B3 may be value of study of tumor proliferation/survival mechanism and contributes to diagnosis and treatment of diverse diseases. Cellular ＆ Molecular Immunology.
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| 关 键 词： | 单细胞克隆 骨髓瘤 治疗 临床表现 |
| 修稿时间： | 2007-04-172007-06-08 |
A novel anti-human syndecan-1 (CD138) monoclonal antibody 4B3: characterization and application |
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| Sun Wan Ping,Wang Feng Ming,Xie Fang,Wang Guo Qing,Sun Jin,Yu Ge Hua,Qiu Yu Hua,Zhang Xue Guang. A novel anti-human syndecan-1 (CD138) monoclonal antibody 4B3: characterization and application[J]. Cellular & molecular immunology, 2007, 4(3): 209-214 |
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| Authors: | Sun Wan Ping Wang Feng Ming Xie Fang Wang Guo Qing Sun Jin Yu Ge Hua Qiu Yu Hua Zhang Xue Guang |
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| Affiliation: | Institute of Biotechnology and Clinical Immunology, Research Laboratory of Jiangsu Province, Soochow University, Suzhou, China. |
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| Abstract: | Syndecan-1 (CD138), a member of integral membrane heparin sulfate proteoglycans, is an essential matrix receptor for maintaining the normal morphological phenotypes. In this study, we generated a specific mouse anti-human syndecan-1 monoclonal antibody (mAb) 4B3 and identified it by competition assay with the available syndecan-1 mAb (BB4). Stained by 4B3, the expression of syndecan-1 was detected on tumor cell lines, such as 8226, U266, XG-1, XG-2, Daudi and Jurkat. The expression was also found on neuron stem cells. It was established that 4B3 mAb could inhibit XG-1 and XG-2 proliferation. The data not only determined that 4B3 mAb was a functional anti-human syndecan-1 mAb, but also indicated that syndecan-1 might be a valuable surface antigen and play an important role in regulation of tumor pathology and differentiation of neural stem cells. This novel antibody 4B3 may be value of study of tumor proliferation/survival mechanism and contributes to diagnosis and treatment of diverse diseases. |
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| Keywords: | syndecan-1(CD 138) monoclonal antibody multiple myeloma |
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