首页 | 本学科首页   官方微博 | 高级检索  
     


Novel cassette array in a class 1 integron in clinical isolates of Acinetobacter baumannii from central Iran
Authors:Alireza Japoni-Nejad  Shohreh Farshad  Alex van Belkum  Ehsanollah Ghaznavi-Rad
Affiliation:1. Student Research Committee, Arak University of Medical Sciences, Arak, Iran;2. Department of Microbiology and Immunology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran;3. Professor Alborzi Clinical Microbiology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran;4. Erasmus MC, Department of Medical Microbiology and Infectious Diseases, ‘s Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands;5. BioMérieux, La Balme les Grottes, France
Abstract:
Antibiotic resistance in Acinetobacter baumannii is a major problem in the hospital and outbreaks caused by this organism have been reported frequently. The present study aimed at determining the antibiotic susceptibility patterns, the prevalence of different classes of integrons and the characterization of integron class 1 gene cassettes in Iranian A. baumannii isolates. A total of 63 non-duplicate A. baumannii isolates were collected from clinical and environmental specimens in the Vali-Asr hospital in the central province of Iran (March to September, 2011). The antimicrobial susceptibility for 15 antibiotics which are used conventionally was determined by disk diffusion. The presence of different integron classes was investigated by PCR and the size of gene cassettes in class 1 integrons was then determined by PCR as well. Moreover, integron cassette arrays of isolates were delineated by RFLP and sequencing amplicons with different lengths. Of 63 isolates 62 (98.4%) carried a class 1 integron. The prevalence of IntI2 was 15.9% and the length of the amplicons ranged from 500 bp to 3 kb. Sequencing of integrons of class 1 revealed the presence of many resistance genes (aadA, aacA, aacC, dfrA, blaGES and blaIMP). We identified a completely new gene cassette which contained aacA7-qacF-aadA5-blaIMP, this cassette has not been reported previously in A. baumannii.
Keywords:A. baumannii   Antimicrobial resistance   Class 1 integron   Gene cassette array   qacF gene
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号