HPLC法测定米铂白蛋白结合型纳米制剂中米铂的含量

目的 建立米铂白蛋白结合型纳米制剂中米铂含量测定的高效液相色谱(HPLC)法.方法 采用HPLC测定米铂白蛋白结合型纳米制剂中米铂的含量.色谱条件:辛烷基硅烷键合硅胶色谱柱(250 mm×4.6 mm,5μm),甲醇-乙腈-水(91:1:8)洗脱,流速1.0 ml/min,检测波长210 nm,柱温30℃,进样量20μ...

Determination of human serum albumin-bound miriplatin by HPLC

Objective To establish a high performance liquid chromatography(HPLC)for the quantification of miriplatin in human serum albumin-bound miriplatin nanoparticle(HSA-miriplatin).Methods The analysis was performed on an octylsilane bonded silica column(250 mm×4.6 mm,5μm)at the flow rate of 1.0 ml/min with SPD detector at 210 nm.The mobile phase consisted of methanol,acetonitrile and water(91:1:8).Results Free miriplatin was extracted from HSA-miriplatin by methanol,and the linear range of miriplatin was 3.63-130.80μg/ml with a correlation coefficient of r=0.9995.The average recovery rates were(99.46±0.24)%,(99.20±1.38)%,(98.30±0.26)%(n=3)at low,medium,high concentrations,respectively.The relative standard deviation(RSD)values reflecting inter-day and intra-day precision were 1.62%(n=6)and 1.32%(n=6),respectively.The method was robust with slight change of flow rate(0.99-1.01 ml/min)and column temperature(28-32℃)with RSD of 1.01%(n=9)and 1.14%(n=9),respectively.Conclusion A simple,selective,sensitive and robust HPLC method is established for determination of miriplatin loaded in HSA-miriplatin.