| 3-硝基丙酸的高效液相色谱分析 |
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| 引用本文： | 江涛 张庆林. 3-硝基丙酸的高效液相色谱分析[J]. 卫生研究, 1999, 28(5): 300-302 |
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| 作者姓名： | 江涛 张庆林 |
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| 作者单位： | 卫生部食品卫生监督检验所!北京100021 |
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| 摘 要： | 利用反相高效液相色谱法定量分析米曲霉菌种培养基中３ 硝基丙酸的含量。样品经乙酸乙酯提取、净化后，以 Ｈｙｐｅｒｓｉｌ Ｃ１８为色谱柱，以 Ｃ Ｈ３ Ｃ Ｎ／ Ｋ Ｈ２ Ｐ Ｏ４ （００２ ｍ ｏｌ／ Ｌ，ｐ Ｈ３０）（１／３，ｖ／ｖ）为流动相，流速为１０ｍ ｌ／ｍ ｉｎ，紫外检测器波长２１０ｎｍ 。该方法最低检出量为１０μｇ／ｋｇ（信噪比为３），回收率为９０１％ ～９８０％ 。
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| 关 键 词： | 3_硝基丙酸 高效液相色谱法 米曲霉 |
Quantitative determination of 3-nitropropionic acid by HPLC] |
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| T Jiang,Q Zhang,X Luo. Quantitative determination of 3-nitropropionic acid by HPLC][J]. Journal of hygiene research, 1999, 28(5): 300-302 |
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| Authors: | T Jiang Q Zhang X Luo |
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| Affiliation: | Institute of Food Safety Control and Inspection, Ministry of Public Health China, Beijing 100021, China. |
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| Abstract: | A reversed-phase liquid chromatographic method to determine the concentration of 3-nitropropionic acid (3-NPA) in the culture medium with Aspergillus oryzae was developed. The culture medium samples were extracted with ethyl acetate. The analytic column was Hypersil C18 and the mobile phase was CH3CN/KH2PO4(0.02 mol/L, pH 3.0) (1/3, v/v). The sample was detected at UV210 nm. The detection limit of this method was at 10 micrograms/kg level and recoveries were 90.1%-98.0. |
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