缺血再灌注损伤对肝脏免疫原性的影响

目的 观察缺血再灌注损伤对肝脏免疫原性的影响.方法 30只大鼠均分为3组,利用免疫荧光及Western blol检测缺血30min再灌注组及缺血60min再灌注组及对照组的肝脏中共刺激分子B7蛋白的表达水平.结果 正常肝脏B7-1、B7-2表达处于极低水平(0.035±0.005、0.025±0.004),缺血再灌注后肝脏B7-1、B7-2的蛋白表达明显升高(P<0.01),缺血再灌注60min组的B7-1、B7-2的表达水平明显高于缺血30min再灌注组(B7-1:0.070±0.017比0.051±0.008,B7-2:0.042±0.004.比0.037±0.004,P<0.01).结论 缺血再灌注损伤使共刺激分子B7蛋白表达升高,提示缺血再灌注损伤可使移植肝免疫原性升高,这些均能促进急性排斥反应的发生.

Effects of ischemia/reperfusion injury on immunogenicity of the liver in rats

Objective To investigate the effect of ischemia/reperfusion (I/R) injury on immunogenicity of the liver. Methods Thirty rats were randomly divided into three groups. The levels of B7 were assayed by using immunochemistry and Western blotting in the 30 min I/R group, 60-min I/R group, and control group. Results The levels of B7-1 and B7-2 were very low in normal liver tissues (0.035 ± 0. 005, 0. 025 ± 0. 004) , but markedly increased after I/R injury ( P < 0.01). The levels of B7-1 and B7-2 in the 60 min I/R group were higher than those in the 30 min I/R group (B7-1:0. 070 ± 0. 017 vs 0. 051 ± 0. 008, B7-2:0. 042 ± 0.004 vs 0. 037 ± 0.004; both P<0.01). Conclusion I/R injury increased the immunogenicity of the liver by up-regulating the costimulatory molecule B7, promoting the development of acute rejection.