Quantitative enzyme-linked immunosorbent assay (ELISA) for hirudin |
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| Authors: | S Spinner G St?ffler E Fink |
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| Affiliation: | 1. Texas Children''s Hospital and Baylor College of Medicine, United States;2. Medical University of South Carolina, United States |
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| Abstract: | A competitive ELISA assay has been developed that permits reproducible quantitation of the anticoagulant hirudin in buffer and urine. Coupling of peroxidase and hirudin was performed with the heterobifunctional reagent N-succinimidyl-3-(2-pyridyldithio)-propionate. In both solvents the lower limit of sensitivity was 8 ng hirudin/ml (0.08 AT-U) while the upper limit was 7.7 micrograms/ml (78.45 AT-U). |
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