纯钛阳极氧化伴水热处理后羟基磷灰石薄涂层种植体的实验研究

目的：了解阳极氧化伴水热处理后纯钛种植的体内成骨效应。方法：36枚纯钛种植体采用4种表面处理，随机植入12只兔股骨内，分别在术后4周、8周、16周取出带种植体骨块制作磨片，观察界面新生骨情况以及抛光、水热处理种植体术后8周的界面超微结构，行表面能谱分析。结果：术后8周，阳极氧化伴水热处理种植体表面编码骨的转化和成熟较快，至16周时界面几乎无编织骨和剥脱的羟基磷灰石碎片，种植体表面的钙，磷含量在种植后增加，抛光种植体表面钙、磷含量增加不明显。结论：纯钛经阳极氧化伴水热处理后，可以加快种植表现编织骨转化为板层骨，从而可能促进种植区的早期愈合，薄涂层特有的优越性尚待进一步研究。

Study on anodic oxidation and hydrothermal treatment of titanium implants with thin hydroxyapatite layers: an implantational test in rabbits

OBJECTIVE: To learn bone response to commercially pure titanium implants with anodic oxidation followed by hydrothermal treatment in vivo. METHODS: Tweleve rabbits were averagely divided into three groups. And 36 implants (9 polished, 9 roughened by grit-blasting, 9 treated with anodic oxidation followed by heat treatment and 9 treated with anodic oxidation followed by hydrothermal treatment) were randomly implanted into femur bones of each group rabbits. Implants were harvested at 4 W, 8 W and 16 W respectively. Slice about 30 microns thick were ground with a grinding machine and stained with 1% toluidine blue. The bone-implant interface was observed with light microscopy and scanning electron microscopy. Implant surface element contents were also detected with EDAX. RESULTS: Woven bone rapidly transferred to lamellae bone at 8 weeks for anodic oxidized implants followed by hydrothermal treatment, which appeared little woven bone and no hydroxyapatite debris between the interfacial zone at 16 weeks. In addition, Ca and P contents increased much more for hydrothermal treated implants after implantation compared with polished implants. CONCLUSIONS: Anodic oxidation followed with hydrothermal treatment of a titanium fixture could speed up woven bone transferring the lamellae bone at early time of implantation, which meant thin HA layers could accelerate bone healing at the implanted site and might shorten healing time. This superiority need more research in the future.