新生儿中性粒细胞CDllb表达的研究

目的 在分子水平上了解新生儿中性粒细胞的功能状态。方法 采用全血流式细胞术和直接免疫荧光法检测了33例正常足月新生儿中性粒细胞CD11b平均荧光强度（MFI）。10例健康成人作对照。结果 自然分娩脐血和成人静脉血中性粒细胞CD11b MFI均低于择期剖宫产脐血和新生儿静脉血（P均＜0．05），自然分娩脐血和成人静脉血间CD11b MFI差异无显著性（P＞0．05）。结论 分娩方式影响新生儿中性粒细胞CD11b表达。足月儿中性粒细胞静息状态下CD11b表达已基本成熟。

Neutrophil CD11b expression in healthy neonates

Objective To explore neutrophil functions of neonates at the molecular level.Methods Neutrophil CD11b median fluorescence intensity(MFI) in 33 normal full term neonates was measured by using whole blood method with flow cytometry and dir ect immunofluorescence technique. 10 healthy adults were measured as controls.Results The neutrophil CD11b MFI in cord blood of vaginally delivered (VD) or in adult venous blood was much lower than that in cord blood of elective caesarian section (ECS) or in neonatal venous blood (all P<0.05). There was no significant difference in CD11b MFI between VD cord blood and adult venous blood (P>0.05).Conclusions 1.The mode of delivery affects neutrophil CD11b expression of neonates.2.CD11b expression on resting neutrophils of full term neonates is mature.