木苏丸防治二甲基亚硝胺诱导的大鼠肝纤维化的实验研究

目的　观察木苏丸防治大鼠肝纤维化的疗效并探讨其作用机制。方法　采用 1%二甲基亚硝胺 (DMN) 10mg/ (kg·d)腹腔注射复制大鼠肝纤维化模型 ,木苏丸治疗组予木苏丸3.12 5 g/ (kg·d)灌胃 ,观察肝功能、血透明质酸 (HA)、超氧化物歧化酶 (SOD)、丙二醛 (MDA)含量变化及基质金属蛋白酶 - 2 (MMP - 2 )、金属蛋白酶组织抑制因子 - 1(TIMP - 1)、α -平滑肌肌动蛋白 (α -SMA)在肝组织中的表达 ;并观察肝脏显微结构改变。结果　木苏丸治疗组与模型对照组比较 ,碱性磷酸酶 (ALP)、MDA、HA明显下降 (P <0 .0 1和P <0 .0 5 ) ,血清白蛋白(ALB)、SOD明显增高 (P <0 .0 1) ,TIMP - 1表达下降 (P <0 .0 5 ) ,MMP - 2的表达无明显改变(P >0 .0 5 ) ;光镜下肝纤维化程度减轻。结论　木苏丸具有保肝、延缓肝纤维化形成的作用 ,其作用机制可能与其抗氧化及其下调TIMP - 1的表达有关

Experimental study of Musu pill on dimethylnitrosamine-induced rat hepatic fibrosis

Objective It is to observe the curative effect of Mu su pill on rat hepatic fibrosis and to discuss its action mechanism.Methods The rat hepatic fibrosis model was made with 1% dimethylni trosamine (DMN ) 10 mg/(kg·d) intraperitoneal injection. Musu pill treatment group was treated with Musu pill 3.125 g/(kg·d) intragastric administration. The content changes o f hepatic function, blood hyaluronate acid (HA), superoxide dismutes (SOD) and m alondialdehyde (MDA), the expressions of matrix metalloproteinase-2 (MMP-2), tis sue inhibitor of metalloproteinase-1 (TIMP-1) and α-smooth-muscle actin (α-SM A), and the hepatic microstructure change were observed.Results Musu pill treatment group compared with model control grou p, the alkalin e phosphatase (ALP), MDA and HA lowered obviously (P<0.01 and P<0.05), the serum albumin (ALB) and SOD heightened obviously (P<0.01), the TIMP-1 expression l ower ed (P<0.05), the MMP-2 expression was unchanged (P>05), and the hepat ic fibrosis degree lightened under light microscope.Conclusion Musu pill has the action of protecting liver and delayi ng hepatic fib rosis formation, the mechanism may be related with its antioxidation and lowerin g the TIMP-1 expression.