黑龙江省肾综合征出血热患者血清汉坦病毒致病株分析

目的 对黑龙江省肾综合征出血热(HFRS)患者血清中汉坦病毒(HV)进行分离、扩增,寻找其与国际标准株(76-118株)之间的差异.方法 应用反转录-巢式-聚合酶链反应(RT-nested-PCR),对50例发病不同时期的HFRS患者血清中HV进行分离、扩增,并将扩增产物进行序列测定及同源性分析.结果 发病7 d内患者血清HV检出率为36.36%(8/22),发病8～14 d的患者血清HV检出率为13.04%(3/23),发病15 d以上的5例患者均未检出.选取的7例样本(第1、9、18、31、37、38、44号标本)HV S基因片段序列与76-118株S基因片段的同源性分别为90.24%、86.72%、89.97%、89.16%、86.45%、87.26%和89.43%.结论 发病7 d内患者检出率明显高于其他时期;黑龙江省HV致病株与国际标准株存在一定差异,说明HV除具有宿主依赖性外,还具有地域的影响.

Analysis on Hantaan virus in hemorrhagic fever patients with renal syndrome in Heilongjiang

Objective To separate and amplify Hantaan virus(HV)in serum of hemorrhagic fever patients with renal syndrome(HFRS)in Heilongjiang,and look for its difference from intemational standard type strain(76-118strains).Methods HVs of different phase in the 8erum of 50 HFRS patients were separated and amplified by RTnested-PCR,its products were analyzed the amplified by sequencing.Results Detectable rate of HV in the patients serum was 36.36%(8/22)in 7 days after onset,it Was 13.04%(3/23)in patients having an onset 8 days to 14 days earlier,5 cases were not detectable 15 days after onset.Comparing the sequence of HV S gene fragment,sample 1,9,18,31,37,38,44 strain had a homology of 90.24%,86.72%,89.97%,89.16%,86.45%,87.26%and 89.43%to 76-118 strains,respectively.Conclusions The positive rate is the highset in 7 days after onset.Nucleotide sequence difference exists between pathogenic strain of Heilongjiang's HV and international standard strain,indicating that not only hosts but also locations can affect HV.