左卡尼汀对血清饥饿诱导心肌细胞凋亡的保护作用

摘要：目的研究左卡尼汀对血清饥饿诱导心肌细胞凋亡的保护作用。方法新生大鼠心肌细胞分为正常组、模型组和不同剂量左卡尼汀（20、40、80μg／mL）治疗组。MTF法测定细胞存活率，流式细胞仪测心肌细胞的凋亡指数，Wsetemblot法检测心肌细胞内半胱氨酸天冬氨酸蛋白酶-3（caspase-3）表达水平。结果左卡尼汀明显抑制血清饥饿诱导的心肌细胞凋亡，表现为细胞存活率升高，凋亡率下降，caspase-3表达降低。结论左卡尼汀对血清饥饿诱导的心肌细胞凋亡具有一定的保护作用。

The Protective Effect of L -Carnitine on Cardiomyocytes Apoptosis Induced by Serum Deprivation

Objective To explore the protective effect of L - carnitine on serum deprivation - induced apoptosis of neo- natal cardiac myocytes. Methods Cuhured neonatal cardiomyocytes were divided into normal group, model group and treat- ment group with different doses of L - carnitine （ 20, 40, 80μg/mL）. The cell viability was determined by MT＇F assay. Ap- optosis index was determined by flow cytometry. Caspase - 3 was investigated by western blot. Results L - carnitine showed the fountion of protective effect expressed as increased cell viability and decreased apoptosis index and caspase - 3 expression. Conclusion L- carnitine has the protective effect on cardiomyocytes apoptosis induced by serum deprivation.