化疗对食管癌患者外周血CD4+CD25+调节性T细胞的影响

目的  探讨化疗对食管癌术后患者外周血CD4+CD25+调节性T细胞的影响。方法  随机选择35例食管癌术后化疗患者，应用流式细胞仪检测其化疗前、化疗1周后外周血CD4+CD25+调节性T细胞的比例，并进行比较，选择30例健康志愿者作为对照。结果  食管癌患者术后化疗前外周血中CD4+CD25+调节性T细胞的比例(10.44±1.45)%高于同期健康对照组（9.35±1.41）%，差异有统计学意义（t=3.059,P＜0.05）。35例患者其化疗前及化疗1周后外周血CD4+CD25+调节性T细胞比例分别为：(10.44±1.45)%、(11.67±1.56)%，两者比较差异具有统计学意义（t=6.342,P＜0.01）。结论  化疗可增高食管癌术后患者外周血CD4+CD25+调节性T细胞的比例。

The impact of chemotherapy on CD4+CD25+ regulatory T cells in peripheral blood of the postoperative patients with esophageal cancer

Objective  To investigate the impact of chemotherapy on CD4+CD25+ regulatory T cells in peripheral blood of the postoperative patients with esophageal cancer. Methods  35 postoperative esophageal cancer patients undergoing chemotherapy were randomly selected. Proportions of CD4+CD25+ regulatory T cells in peripheral blood of the patients were detected using FACSCalibur (FACScan) before and 1 week after chemotherapy, and were compared with those of 30 healthy volunteers who were randomly selected as the control group. Results  Proportions of CD4+CD25+ regulatory T cells in peripheral blood of the 30 healthy volunteers and 35 patients before and 1 week after chemotherapy were （9.35±1.41）% vs (10.44±1.45)% and (10.44±1.45)% vs (11.67±1.56)% respectively，and there were significant differences between them（t=3.059,P＜0.05 and t=6.342,P＜0.01）. Conclusion  Chemotherapy could increase the proportion of CD4+CD25+ regulatory T cells in peripheral blood of the postoperative patients with esophageal cancer.