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| 苯并[a]芘诱导内皮细胞细胞色素P4501A1表达的研究 | |
| 引用本文： | 杨进波,蒋长征,柯磊,徐谦,杨杪,贺涵贞,胡丙长,邬堂春. 苯并[a]芘诱导内皮细胞细胞色素P4501A1表达的研究[J]. 中华劳动卫生职业病杂志, 2003, 21(5): 362-364 |
| 作者姓名： | 杨进波 蒋长征 柯磊 徐谦 杨杪 贺涵贞 胡丙长 邬堂春 |
| 作者单位： | 1. 430030,武汉,华中科技大学同济医学院劳动卫生与环境卫生学系 2. 美国哈佛大学公共卫生学院 |
| 基金项目： | 国家自然科学基金海外青年学者合作研究基金资助项目(3 0 12 80 2 1)，高等学校优秀青年教师教学科研奖励计划资助项目(2 0 0 1)，国家重点基础研究规化项目(2 0 0 2CB5 12 90 5 ) |
| 摘 要： | 目的：探讨苯并[a]芘(BaP)诱导猪主动脉内皮细胞细胞色素P4501A1(CYP1A1)表达及活性的影响。方法：离体培养猪主动脉内皮细胞，不同浓度BaP(0、0．5、1．0、5．0、10．0μmol／L)染毒24h，分别以Western-blot法和免疫组化方法检测不同浓度BaP诱导内皮细胞合成CYP1A1的影响，同时还探讨了乙氧基异吩噁唑-o-去乙氧基酶(EROD)活力的变化规律。结果：Western-blot法未能检测出对照组CYP1A1的表达，而各染毒组均检出了CYP1A1的表达；免疫组化结果显示染毒组仅在部分内皮细胞呈阳性反应；EROD活力诱导高峰的BaP浓度为0．5～1.0μmol／L。结论：BaP可诱导部分猪主动脉内皮细胞合成CYP1A1；EROD活力诱导高峰的BaP浓度为0．5～1．0μmol／L。 |
| 关 键 词： | 苯并[a]芘 诱导 内皮细胞 细胞色素 P4501A1 表达 研究 |
| 修稿时间： | 2003-02-27 |
The expression of cytochrome P450 1A1 of endothelial cells induced by benzo(a) pyrene |
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| YANG Jin bo ,JIANG Chang zheng,KE Lei,XU Qian,YANG Miao,HE Han zhen,HU Bing chang,WU Tang chun. The expression of cytochrome P450 1A1 of endothelial cells induced by benzo(a) pyrene[J]. Chinese journal of industrial hygiene and occupational diseases, 2003, 21(5): 362-364 | |
| Authors: | YANG Jin bo JIANG Chang zheng KE Lei XU Qian YANG Miao HE Han zhen HU Bing chang WU Tang chun |
| Affiliation: | Department of Labor Health and Environment Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. |
| Abstract: | OBJECTIVE: To explore the effect of benzo(a)pyrene (BaP) on the expression and the activities of cytochrome P450 1A1 (CYP1A1) of porcine aortic endothelial cells. METHODS: Porcine aortic endothelial cells were cultured in vitro, and treated with different concentrations of BaP (0, 0.5, 1.0, 5.0, 10.0 micro mol/L) for 24 hours, CYP1A1 expression was determined by Western blot and immunohistochemistry. At the same time, the ethoxyresorufin-o-deethylase (EROD) activities were measured by spectrofluorometer. RESULTS: By Western blot, the expression of CYP1A1 of control cells was not found, but the expression of CYP1A1 of cells treated with BaP was found; By immunohistochemistry, only part of endothelial cells treated with BaP had positive expression of CYP1A1. The peak activities of EROD induced by BaP was at the concentration of 0.5 - 1.0 micro mol/L. CONCLUSION: BaP could induce part of endothelial cells to synthesize CYP1A1. BaP of 0.5 - 1.0 micro mol/L could induce peak activities of EROD. |
| Keywords: | Benzo(a)pyrene Endothelial cells Cytochrome P450 1A1 |
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