人胰腺癌细胞CFPAC-1裸小鼠原位移植瘤模型的建立

目的:建立人胰腺癌裸小鼠原位移植瘤模型,并对其生物学特性进行研究.方法:将人胰腺癌细胞CFPAC-1接种于裸鼠皮下,并将皮下肿瘤组织原位移植于裸鼠胰腺,比较两种移植瘤的生长及转移情况.结果:胰腺癌皮下移植瘤模型与胰腺癌原位移植瘤模型的体内成瘤率、生长情况及形态学上均无显著差异.皮下移植瘤模型呈局限性生长,无一例发生远处转移;原位移植瘤模型远处转移率高达87.5%,发生75.0%腹膜转移,37.5%肝转移,12.5%肺转移,37.5%淋巴结转移,并保持分泌CA19-9的特性.结论:人胰腺癌裸小鼠原位种植瘤模型明显优于皮下移植瘤模型,较好地模拟了人胰腺癌自然生长和局部浸润及远处转移过程,是胰腺癌体内研究理想的动物模型.

Establishment of orthotopic transplantation nude mouse model with pancreatic cancer celllines CFPAC-1

Objective To establish the orthotopic transplantation nude mouse model of pancreatic cancer for the study of its biological characteristics. Methods The subcutaneous tumors made by the subcutaneous injection of pancreatic cancer cell lines CFPAC-1 were used as the source of tissue for orthotopic implantation of tissue onto the pancreas, then the survival rates and spontaneous metastatic rate were compared with the two groups. Results There was no significant deviation in the rate of success, growth status and morphology between the two groups. The subcutaneous tumors were not metastasized, but the orthotopic implanted tumor metastatic rate, peritoneum metastatic rate, hepatic metastatic rate,pulmonary metastatic rate and lymph node metastatic rate were 87.5% ,75.0% ,37.5%, 12.5% and 37.5% respectively. The orthotopic implanted tumor secreted CA19-9 as well. Conclusions The orthotopic implanted tumor model, more likely to mimic the process of tumor growth, local infiltration and metastasis, is superior to the subcutaneous implanted tumor model, thus it is ideal for the study of pancreatic cancer in vivo.