日本血吸虫未成熟卵可溶性抗原的初步分析

采用ＳＤＳ－ＰＡＧＥ和ＥＬＩＢ技术分析了日本血吸虫未成熟卵可溶性抗原（ＳＩＥＡ），并与可溶性成熟卵抗原（ＳＥＡ）进行比较，二者主要蛋白区带存在明显差异，同时，ＥＬＩＢ结果表明，ＳＩＥＡ中感染血清和免疫血清所识别的６５ｋＤａ、６２ｋＤａ、５０ｋＤａ、２８ｋＤａ和和２６ｋＤａ等抗原组分不出现于ＳＥＡ，推测其在ＳＩＥＡ诱导宿主产生抗雌虫生殖及抗卵胚发育免疫应答方面起作用。

Preliminary Analysis of the Soluble Immature Egg Antigen of Schistosoma japonicum by SDS-PAGE and ELIB

This paper reports that the protein compositions of soluble immature egg antigen(SIEA) and soluble egg antigenSEA)was analyzed by SDS-PAGE.Silver staining of the two antigens following SDS-PAGE demonstrated that the major band pattern was different from each other.Enzyme linked immune blotting(ELIB) analysis of SIEA and SEA with infected and immunized rabbit sera also showed a significant difference between the two antigens with the 67kDa,62kDa,54kDa,50kDa,28kDa and 2 6kDa antigenic molecules specially recognized by the two antisera only in SIEA but not in SEA.In view of both antifecundity and anti-embryonation immunity being induced by SIEA but not by SEA,the above mentioned antigenic molecules identified in SIEA may be involved in the in duction of the immunity against the egg production and development of Schistosoma japonicum.