髓磷脂碱性蛋白促进单个核细胞对内皮细胞的粘附效应

目的：探讨ＭＢＰ对外周血单个核细胞（ＰＢＭＮＣ）与人脐静脉内皮细胞（ＨＵＶＥＣ）粘附性的影响，以揭示中枢神经系统（ＣＮＳ）炎症时ＰＢＭＮＣ进入ＣＮＳ的可能原因。方法：用细胞粘附试验，研究ＰＢＭＮＣ与ＨＵＶＥＣ的粘附性，用细胞免疫化学法、ＦＡＣＳ分别观察ＶＣＡＭ－１和ＩＣＡＭ－１的表达。结果：ＭＢＰ活化的ＰＢＭＮＣ与ＭＢＰ刺激的ＰＢＭＮＣ培养上清作用的ＨＵＶＥＣ的粘附性较对照有显著提高。ＩＣＡＭ＿１

Myelin basic protein promote the effect of peripheral blood mononuclear cells adhesion to human umbilical vein endothelial cells

Objective:To study the influence of MBP to the adhesion of PBMNC to HUVEC,in order to explore possible reason of the PBMNC entering CNS in CNS inflammation.Methods:The adhesion test of cell was used to study the adhesion of PBMNC to HUVEC.Immunocytochemistry and FACS were used to test the expression of ICAM 1 and VCAM 1.Results:Compared with control,much more adhesion cells were showed during PBMNC stimulated by MBP adhesion to HUVEC culturing with the supernatant of MBP stimulating PBMNC.Either anti ICAM 1 McAb or sVCAM 1 can inhibit the PBMNC HUVEC adhesion.Furthermore,the supernatant can induce VCAM 1 expression and improve the amount of ICAM 1 on HUVEC.Meanwhile,it was showed that MBP can stimulate PBMNC to secrete TNF.TNF can induce the expression of ICAM 1 and VCAM 1,the latter can promote PBMNC HUVEC adhesion.Conclusion:MBP can promote the adhesion of PBMNC to HUVEC and may play an important role during PBMNC infiltration to CNS in CNS inflammation.