人子宫内膜基质细胞和腺体细胞的分离培养及鉴定

目的：人子宫内膜分离培工得到纯度较高的内膜基质细胞和腺体细胞。方法：采用二次滤网过滤法进行分离并通过光镜观察和免疫细胞化学染色对其进行鉴别。结果：基质细胞在接种后0．5h开始贴壁，可见梭形和多角形两种形态的细胞。免疫细胞化学染色显示这两种细胞均具有波形蛋白免疫反应，反应率可达95％以上，而细胞角蛋白无免疫反应性，提示它们为来源于内膜基质的基质细胞，大部分腺体细胞在接种24h后贴壁，培养后4d左右腺体细胞呈旋涡状排列，单个细胞为多角形，核大而圆，腺细胞呈细胞角蛋白反应性，反应率在90％以上，结论：采用二次滤网过滤法可成功地分离入人子宫内膜基质细胞和腺体细胞。

ISOLATION,CULTURE AND VERIFICATION OF STROMAL AND GLANDULAR CELLS OF HUMAN ENDOMETRIUM

Objective Stromal and glandular cells of human endometrium were successfully isolated and cultured in this experiment. Methods These two types of cells were observed by light microscopy and verified by immunocytochemical staining. Results The result showed that some of stromal cells became adherent after.0 5h of culture.Two shapes of stromal cells were found.One was spindle and the another was polygonal.Immunocytochemical staining method showed that both shapes of stromal cells were positive for vimentin,with 95% of positive rate,and negative for cytokeratin 1,indicating that these cells were endometrial stromal cells.Most of glandular cells became adherent after 24h of culture and formed tightly packed whorls after 4d.Individual cells were polygonal and had a large and round nucleus.Immunocytochemical staining method showed that glandular epithelial cells were positive for cytokeratin 1,with 90% of positive rate,suggesting their nature of epithelial cells. Conclusion Stromal and glandular cells of human endometrium were successfully isolated by using two series of filter and cultured in this experiment. [