| 家兔房水及角膜中妥布霉素的测定 |
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| 引用本文： | 郭霞.家兔房水及角膜中妥布霉素的测定[J].抗感染药学,2011,8(3):176-179. |
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| 作者姓名： | 郭霞 |
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| 作者单位： | 苏州市立医院东区,江苏苏州,215001 |
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| 摘 要： | 目的:建立妥布霉素-地塞米松滴眼液在不同时间点时家兔眼房水及角膜中妥布霉素的HPLC-ELSD法测定。方法:家兔给予妥布霉素-地塞米松滴眼液后分别于5,10,20,30,60,90,120min时抽取家兔眼房水,取出角膜,经处理后采用HPLC-ELSD法检测房水及角膜内的妥布霉素质量。结果:妥布霉素在62.50～800.00μg/mL或0.062.5～8.00mg/g范围内,峰面积响应值(A)的对数与质量浓度(μg/mL)的对数呈现良好的线性关系,在房水及角膜中相关系数(r)分别为0.9994和0.9991,平均回收率分别为100.48%(RSD=1.81%,n=5),102.13%(RSD=1.58%,n=5);给药20min左右兔房水及角膜中的药物浓度峰值分别为291.70μg/mL和2.842mg/g。结论:本法灵敏、准确,适用于测定兔眼房水、角膜中妥布霉素的质量浓度的测定。
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| 关 键 词： | 家兔 妥布霉素-地塞米松滴眼液 房水 角膜 |
Determination of Tobramycin in Intraocular Aqueous and Cornea of Rabbit by HPLC |
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| GUO Xia.Determination of Tobramycin in Intraocular Aqueous and Cornea of Rabbit by HPLC[J].Anti-infection Pharmacy,2011,8(3):176-179. |
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| Authors: | GUO Xia |
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| Institution: | GUO Xia* Suzhou Municipal Hospital East Area,Suzhou Jiangsu 215001 |
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| Abstract: | Objective:To establish the determination of tobramycin in intraocular aqueous and cornea of rabbit at different time points by HPLC.Methods:After the application of tobramycin-dexamethasone eye drops and by extracting the intraocular aqueous and taking out the cornea of rabbit in 5,10,20,30,60,90,120 min respectively,the mass concentrations of tobramycin in intraocular aqueous and cornea were determined by HPLC-ELSD.Results:The logarithm of peak area response value and the logarithm of mass concentration pr... |
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| Keywords: | rabbit tobramycin-dexamethasone eye drops aqueous corneal |
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