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| 雌激素对内质网应激介导的大鼠脑缺血再灌注损伤的保护作用 | |
| 引用本文： | 杨丽娟,马中女,张慧,刘红霞. 雌激素对内质网应激介导的大鼠脑缺血再灌注损伤的保护作用[J]. 滨州医学院学报, 2010, 33(4): 253-255. DOI: 10.3969/j.issn.1001-9510.2010.04.005 |
| 作者姓名： | 杨丽娟 马中女 张慧 刘红霞 |
| 作者单位： | 滨州医学院生理学教研室,烟台市,264003;滨州医学院机能学实验室 |
| 基金项目： | 滨州医学院校级科技计划，滨州医学院大学生创新课题 |
| 摘 要： | 目的观察雌激素对大鼠脑缺血/再灌注损伤的保护作用,并进一步探讨内质网应激在其中的作用。方法 250 g左右SD大鼠随机分为假手术组、模型组、雌激素(E2)预处理组,采用大脑中动脉阻塞(MCAO)法建立缺血2 h,再灌注22 h模型;5分法进行神经行为学评分、干湿重法测定脑水肿程度、利用HE染色观察鼠脑组织形态学改变,RT-PCR技术检测GRP78mRNA的表达。结果 (1)假手术组神经行为学评分、组织细胞形态无明显异常,脑组织含水量呈基础水平;模型组神经行为学评分显著升高;HE染色出现明显病理学改变,神经细胞及胶质细胞肿胀,胞核碎裂或消失,部分神经细胞坏死消失,残存神经细胞胞体缩小,胞核固缩,核仁消失,脑水肿明显(P<0.05);E2预处理组与模型组相比神经行为学评分降低,组织病理改变较轻,脑水肿减轻。(2)假手术组鼠脑GRP78 mRNA有一定表达,缺血/再灌注损伤时表达增加,E2预处理可下调其表达。结论雌激素可能通过抑制内质网应激途径减轻大鼠脑缺血/再灌注损伤。 |
| 关 键 词： | 雌激素 脑缺血/再灌注 内质网应激 |
Estrogen protects rat cerebrum against focal cerebral ischem/reperfusion injury through attenuating endoplasmic reticulum stress |
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| YANG Lijuan,MA Zhongnü,ZHANG Hui,LIU Hongxia. Estrogen protects rat cerebrum against focal cerebral ischem/reperfusion injury through attenuating endoplasmic reticulum stress[J]. Journal of Binzhou Medical College, 2010, 33(4): 253-255. DOI: 10.3969/j.issn.1001-9510.2010.04.005 | |
| Authors: | YANG Lijuan MA Zhongnü ZHANG Hui LIU Hongxia |
| Affiliation: | YANG Lijuan1 MA Zhongnü1 ZHANG Hui2 LIU Hongxia11 Department of Physiology,Binzhou Medical University,Yantai 264003,2 Function Laboratory |
| Abstract: | Objective To investigate the protective mechanism of estrogen in SD rats with focal cerebral ischemic/reperfusion injury mediated by endoplasmic reticulum stress(ERS).Methods SD rats were randomly divided into three groups: shame operated group,model group and E2 pretreatment group.The rats in the last two groups were subjected to ischemia for 2 hours followed by 22 hours of reperfusion,there were 10 rats in each group.The modified thread thrombotic method was applied to establish the model of rat with foca... |
| Keywords: | estrogen ischemia/reperfusion endoplasmic reticulum stress |
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