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| 生物素-链霉抗生物素蛋白硷性磷酸酶体系标记检测DNA探针 | |
| 引用本文： | 陈俊杰,游乐然,王若菡,张秋萍. 生物素-链霉抗生物素蛋白硷性磷酸酶体系标记检测DNA探针[J]. 四川大学学报(医学版), 1988, 0(4) |
| 作者姓名： | 陈俊杰 游乐然 王若菡 张秋萍 |
| 作者单位： | 华西医大生物化学教研室(陈俊杰，游乐然，王若菡)，华西医大生物化学教研室(张秋萍) |
| 摘 要： | 本室采用近年发展的生物素—链霉抗生物素蛋白体系检测特异互补的核酸序列,先经缺口翻译制备生物素DNA探针,分子杂交后,再与链霉抗生物素蛋白—生物素—硷性磷酸酶复合物反应和显色,检测限度约50pg靶DNA,并将其结果与~(35)S标记相同的探针进行了比较。 |
| 关 键 词： | 生物素 链霉抗生物素蛋白 脑髓鞘硷性蛋白cDNA 探针 杂交 |
DNA PROBES LABELED AND DETECTED WITH BIOTIN STREPTAVIDIN SYSTEM |
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| Chen Junjie,You Leran,Wang Ruohan,Zhang Qiuping. DNA PROBES LABELED AND DETECTED WITH BIOTIN STREPTAVIDIN SYSTEM[J]. Journal of Sichuan University. Medical science edition, 1988, 0(4) | |
| Authors: | Chen Junjie You Leran Wang Ruohan Zhang Qiuping |
| Affiliation: | Chen Junjie;You Leran;Wang Ruohan;Zhang Qiuping Department of Biochemistry |
| Abstract: | Biotin-labeled DNA probe was prepared bynick translation in the presence of biotiny-lated analog of dCTP and then hybridizedwith complementary DNA. The bound bio-tin-labeled DNA probe was detected withstreptavidin biotinylated alkaline phospha-tase complex. The results of dot blot andSouthern blot transfer were compared withthose obtained with ~(35)S-labeled DNA probeunder similar condition. Detection limits ofthis procedure were near 50 pg of targetDNA. |
| Keywords: | Biotin Streptavidin Brain myelin basic protein cDNA Probe Hybridization |
| 本文献已被 CNKI 等数据库收录！ | |