促红细胞生成素对横纹肌溶解大鼠急性肾衰竭的肾脏保护作用

目的：观察促红细胞生成素（EPO）能否减轻横纹肌溶解引起的急性肾衰竭（ARF）大鼠肾损伤程度并探讨机制。方法：重200g左右SD大鼠48只,禁水14h后分4组,正常对照组：两后腿肌肉注射生理盐水10ml/kg;ARF组：两后腿肌肉内注射50%甘油（10ml/kg）;EPO立即干预组：肌肉注射50%甘油的同时腹腔注射EPO2000μg/kg,12h后重复1次;EPO延迟干预组：在肌肉注射甘油6h后注射EPO2000μg/kg,12h重复1次。观察各组尿量、肾功能和肾组织病理变化。结果：与ARF组相比,EPO立即干预组和延迟干预组血尿素氮、血肌酐浓度均明显降低,ATN评分下降（P〈0.05）。肌肉注射甘油的3组大鼠24h肾组织中均存在明显的小管细胞凋亡,EPO干预组凋亡情况减轻;肌肉注射甘油的大鼠肾组织抗凋亡因子Bcl-XL的蛋白质表达比正常对照组增加,EPO立即干预和延迟干预组增加更加明显。结论：立即注射和延迟6h注射EPO均可减轻甘油诱导的横纹肌溶解性大鼠急性肾损伤。

Erythropoietin Reduce Kidney Injury in Rhabdomyolysis-induced Acute Renal Failure in Rats

Objective：To observe if Erythropoietin （EPO） can reduce kidney injury in rhabdomyolysisinduced acute renal failure（ ARF） in rats and investigate the mechanism. Methods： 48 SD rats were divided into four groups： Normal control group were injected 10 ml/kg saline in hind legs muscle. ARF group were injected 50% glycerol （10 ml/kg, I. M）. EPO immediate group and EPO- delayed group were injected EPO（2000/a/kg） immediately or 6h later in addition to 50% glycerol injected,and repeated after 12h. The urine volume, renal function and kidney histopathology were investigated. Results： Compared with ARF group, EPO treated groups （immediate and 6h delayed） have lower blood urea nitrogen, Serum creatinine and ATN score（P〈0.05）. 24 h kidney tissue of ARF group had obvious tubule cell apoptosis, EPO treated groups had less apoptosis. Western blot showed Bcl- XL protein was increased in ARF group compared normal control group, Bcl - XL protein expressed more in EPO treated groups than ARF group. Conelusion：EPO, not matter was given immediately or 6 h later, can reduce kidney injury in rhabdomyolysis- induced acute renal failure in rats.